微载体培养BHK-21细胞生产狂犬病毒Flury株的研究  

Production of Flury Strain of Rabies Virus by Microcarrier Culture of BHK-21 Cells

作　　者：刘艳霞 Liu Yanxia(Liaoning Yikang Biological Corporation Limited,Liaoning Liaoyang 111000)

机构地区：[1]辽宁益康生物股份有限公司,辽宁辽阳111000

出　　处：《现代畜牧兽医》2018年第9期6-9,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：本文主要研究了利用生物反应器Tide-Cell微载体培养的BHK-21细胞生产狂犬病毒Flury株工艺。总数为1×1010个BHK-21细胞接入装有500 g BioNOCTMⅡ型载体的Tide-Cell培养系统,最适条件下培养120 h。当细胞培养系统中葡萄糖消耗量达到8 g/(L·h)左右,细胞数达到4×1011时,按照MOI为1接入Flury株病毒。一批至少可以收获10次(50 L/次)毒价高于107.5TCID50/mL的抗原液,其中包含5次毒价为108.0TCID50/mL的抗原液。提高抗原液病毒含量有助于提升狂犬病灭活疫苗效价,从而降低生产成本。In this paper, we studied the process of BHK-21 cells cultured in Tide-Cell bioreac - tot filled microcarrier manufacture rabies virus Flury strain. 1×10 10 BHK-21 cells were seeded to bioreactor cell culture system（CCS） bioreactor equipped with 500 g BioNOCTMⅡ vector and cultured optimum parameters. Flury virus（MOI：1） were seeded with 8 g/L·h glucose consumption and 4×10 11 total cells in cell culture system after 120 h. Every time would have harvest 50 L antivirus so- lution（TCID50〉10 7.5/mL） and 10 times for each batch, including antivirus solution TCID50 about 10 8.0/ mL for 5 times. Increase the virus content of the antivirus solution help to improve the efficacy of rabies inactivated vaccine, it contribute toreduce production cost.

关 键 词：微载体 BHK-21细胞 狂犬病毒 Flury株 

分 类 号：S852.65[农业科学—基础兽医学]