健脾软肝方对四氯化碳致肝纤维化大鼠α-SMA及其mRNA表达的影响  

Effects of Jianpi Ruangan Formula on the Expression of α-SMA and m-RNA in Rats with Hepatic fibrosis Induced by Carbon Tetrachloride

作　　者：陈文玲[1] 王宪东 陈文慧[1] 朱晓松[1] 姚政[1] 石安华[1] Chen Wenling;Wang Xiandong;Chen Wenhui;Zhu Xiaosong;Yao Zheng;Shi Anhua(Clinical Medical School,Yunnan University of Traditional Chinese Medicine,Kunming 650000,China;Yunnan Academy of Traditional Chinese Medicine,Kunming 650223,China)

机构地区：[1]云南中医学院,昆明650000 [2]云南省中医中药研究院,昆明650223

出　　处：《世界中医药》2018年第9期2261-2267,2271,共8页World Chinese Medicine

基　　金：云南省科技厅应用基础面上项目(2013FZ091)

摘　　要：目的:明确健脾软肝方对肝纤维化大鼠α-SMA及其mRNA表达的影响,初步探讨健脾软肝方防治肝纤维化的作用机制,为肝纤维化的中医临床治疗提供实验依据。方法:选取Wistar雄性大鼠72只,随机分为正常组、模型组、扶正化瘀胶囊组、健脾软肝方高、中、低剂量组。模型组利用CCl4对大鼠进行腹腔注射,按0.2 m L/100 g腹腔注射30%CCl4与70%橄榄油混合溶液,正常组注射等量生理盐水,2次/周,4周后复制大鼠肝纤维化动物模型,该实验采用免疫组化ABC法检测肝纤维化大鼠α-SMA蛋白的表达水平,采用Real-time PCR法检测肝纤维化大鼠α-SMA mRNA的表达水平,采用H-E染色和Mallory染色观察大鼠肝纤维化的病变程度。结果:模型组α-SMA蛋白表达显著上升(P<0.05);健脾软肝方高、中、低剂量组组与模型组比较,差异有统计学意义(P<0.05),其中高、中、低剂量组之间α-SMA蛋白阳性表达逐渐升高,可以呈一定的药物依赖关系。模型组α-SMA mRNA表达显著上升(P<0.05);健脾软肝方高、中、低剂量组组与模型组比较,差异有统计学意义(P<0.05),且高、中、低剂量组之间α-SMA mRNA表达水平逐渐升高,呈一定的药物依赖关系。结论:健脾软肝方抗肝纤维化的作用机制可能与抑制大鼠肝脏内α-SMA及其mRNA的表达有关。Objective： To study the effects of Jianpi Ruangan Formula on the expression of α-smooth muscle actin （α-SMA） and m-RNA in rats with hepatic fibrosis induced by carbon tetrachloride （CCl 4）,to preliminarily discuss the mechanism of Jianpi Ruangan Formula in preventing and treating liver fibrosis,and to provide experimental basis for clinic treatment of hepatic fibrosis in traditional Chinese medicine. Methods： A total of 72 Wistar male rats were selected and randomly divided into normal group,model group,Fuzheng Huayu Capsule group,Jianpi Ruangan Formula high,middle,low dose group.Rats in model group was intraperitoneally injected with the mixed solution of 30% CCl 4 and 70% olive oil at 0.2 mL/100 g while the normal group was intraperitoneally injected with the same dose of saline.Each group injected twice a week for 4 weeks,and the animal models of rat liver fibrosis was replicated after 4 weeks.The expression of α-SMA in rats with hepatic fibrosis was detected by ABC immunohistochemical method and expression of the m-RNA was determined by Real-time PCR method.The HE staining and Mallory staining were applied to observe the pathological changes of hepatic fibrosis in rats. Results： The expression of α-SMA protein in model group increased （ P 〈0.05）.Compared with the Jianpi Ruangan formula high,middle,low doze groups,the difference was statistically significant （ P 〈0.05）.Among them,the positive expression of α-SMA protein in the high,middle and low dose groups gradually increased,showing some signs of drug dependence.Compared with the Jianpi Ruanfang formula high,middle,low doze groups,the expression of α-SMA mRNA in model group significantly increased （ P 〈0.05）,and there was significant difference （ P 〈0.05）.The expression of α-SMA mRNA in the high,middle and low dose groups gradually increased,showing some signs of drug dependence. Conclusion： The mechanism of the Jianpi Ruangan Formula against hepatic fibrosis may be related to the inhibition of the expression o

关 键 词：健脾软肝方 肝纤维化 Α-平滑肌肌动蛋白 大鼠 四氯化碳 

分 类 号：R285.5[医药卫生—中药学]