核桃腐烂病病原菌的分离与鉴定  

Isolation and identification of pathogen of walnut rot disease

作　　者：张海军 陈春艳[2] 谢映平[2] 田忠科 Zhang Haijun;Chen Chunyan;Xie Yingping;Tian Zhongke(Yangquan Forestry Sciences Research Institute,Yangquan Shanxi 045000,China;College of Life Sciences,Shanxi University,Taiyuan Shanxi 030006,China;Yangquan Applied Technology Institute,Yangquan Shanxi 045000,China)

机构地区：[1]山西省阳泉市林业科学研究所,山西阳泉045000 [2]山西大学生命科学学院,山西太原030006 [3]山西省阳泉市应用技术研究所,山西阳泉045000

出　　处：《中国植保导刊》2018年第9期17-20,共4页China Plant Protection

基　　金：山西省科技攻关项目(20140311017-6)

摘　　要：核桃腐烂病的发生严重影响核桃树的生长与结果。将染病树皮进行培养,划线分离出一株真菌,应用PCR技术对分离菌株的线粒体基因[the small r RNA subunit(rns)]克隆后测序,并将所测序列与美国国家生物技术信息中心(Natinal Center for Biotechnology Information,NCBI)数据库中已有的序列进行分析比较,利用Neighbor-joining建立系统发育树。结果表明,分离出的菌株与茎点霉属(Phoma sp.)聚集在同一支上,可以确定是茎点霉属(Phoma sp.)。Walnut rot was a serious disease which brought damage to growing and fruiting of walnut tree. A strain of fungus was isolated from cultivating infected bark of walnut tree, and its small r RNA subunit was cloned and sequenced by PCR. The sequces were analyzed and compared with the existing sequences in NCBI, and phylogenetic tree was established with Neighbor-joining. The results showed that the isolated strain had high homology with Phoma sp.（97%）,and it was proved that strain was belonged to Phoma sp.

关 键 词：核桃腐烂病 病原真菌 分离 鉴定 茎点霉属 

分 类 号：S436.62[农业科学—农业昆虫与害虫防治]