2014-2016年乌鲁木齐市肠炎沙门菌耐药与分子分型分析  

Drug resistance and molecular typing of Salmonella enteritidis in Urumqi from 2014 to 2016

作　　者：杨毅[1] 王超[1] 陈国利[1] 巴特尔[1] 吴亚英[1] YANG Yi;WANG Chao;CHEN Guoli;Battelle;WU Yaying(Urumqi Center for Disease Control and Prevention,Xinfiang 830000,China)

机构地区：[1]乌鲁木齐市疾病预防控制中心,新疆830000

出　　处：《医学动物防制》2018年第10期962-964,968,共4页Journal of Medical Pest Control

基　　金：新疆维吾尔自治区卫生计生委青年医学科技人才专项(WJWY-201833)

摘　　要：目的对乌鲁木齐市2014-2016年分离的人群肠炎沙门菌株进行耐药性和PFGE分子分型研究。方法收集分离自乌鲁木齐市腹泻病监测哨点的101株肠炎沙门菌,对其进行7类抗生素的药敏测试与PFGE分子分型分析。结果101株肠炎沙门菌中,62株分离自男性,36株分离自女性,0~4岁低龄儿童中共分离62株(62.4%),分离时间集中于每年的5~7月。101株肠炎沙门菌对萘啶酸的耐药率为83.1%,对四环素的耐药率为30.7%,对氨苄西林的耐药率为29.7%;对环丙沙星、庆大霉素、头孢噻肟均比较敏感。所有菌株酶切图谱分为18个PFGE型,分别命名为S-1~S-18;各带型包含菌株数为1~28株不等,S-4、S-7和S-9分别包含28、15和28株菌,为该血清型主要优势带型。结论乌鲁木齐市2014-2016年肠炎沙门菌对抗生素的耐药尚不严重,仍需加强对多重耐药株的监控;加强PFGE优势带型肠炎沙门菌的流行病学调查,降低潜在聚集性疫情的风险。Objective To investigate the antimicrobial susceptibility testing and PFGE typing of clinical isolates of Salmonella enteritidis in Urumqi from 2014 to 2016. Methods 101 strains of Salmonella enteritidis isolated from diarrhea cases were tested by drug sensitivity of 7 kinds antibiotics and PFGE. Results Among 101 strains of Salmonella enteritidis, 62 were isolated from male patients, 36 from female patients. A total of 62 strains Salmonella enteritidis were isolated from young children aged from 0 to 4 years old（62.4%）, the isolated time mainly centralized from May to July of the year. The resistance rate of 101 strains Salmonella enteritidis to nalidixic was 83.1% ; to tetracycline was 30.7%, to ampicillin was 29.7% ; these isolations were sensitive to ciprofloxacin, gentamicin and cefotaxime. All 101 strains of Salmonella enteritidis were divided into 18 molecular patterns by digestion with XbaI and pulsed field gel electrophoresis, each pattern contained 1～28 strains. S-4, S-7 and S-9 were the main PFGE types, including 28, 15 and 28 strains respectively. Conclusion The status of drug resistance of clinical isolates of Salmonella enteritidis in Urumqi were not high, but the surveillance for muhidrug resistance still needs to be strengthened. It is necessary to strengthen the epidemiological investigation of advantage PFGE types, and reduce the potential risk of diarrhea outbreaks.

关 键 词：肠炎沙门菌 药敏试验 脉冲场凝胶电泳 分子分型 

分 类 号：R378.22[医药卫生—病原生物学]