赖氨酸翻译后修饰蛋白质的化学(半)合成及其应用  

Chemical(Semi-) Synthesis and Applications of Lysine Post-Translationally Modified Proteins

作　　者：王志鹏[1,2] 李曼 李珲 刘志华 李影 郑基深 Wang Zhipeng1,2,Li Man3,Li Hui4,Liu Zhihua3,Li Ying1,Zheng Ji-Shen1(a School of Life Sciences, University of Science and Technology of China, Hefei 230027, China;b Department of Chemistry, Texas A＆M University, Texas 77840, USA;c Department of Plant Pathology and Microbiology, Texas A＆M University, Texas 77843, USA;d Anhui Supervision of Veterinay Drug and Feed, Hefei 230091, China)

机构地区：[1]中国科学技术大学生命学院 [2]德州农工大学化学系,美国得克萨斯州77840 [3]德州农工大学植物病理与微生物学系,美国得克萨斯州77843 [4]安徽省兽药饲料监察所

出　　处：《有机化学》2018年第9期2400-2411,共12页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No.U1732161);安徽省杰出青年科学基金(No.1808085J04);合肥物质科学技术中心方向项目培育基金(No.2017FXCX002)资助项目~~

摘　　要：天然蛋白质的翻译后修饰是表观遗传学的重要研究方向之一.最近几年,新型赖氨酸翻译后修饰模式的发现及其对染色体结构和基因转录的重大调控作用是表观遗传学研究重点之一,引起了生物学家的广泛关注.目前发展了一系列含有特定赖氨酸翻译后修饰蛋白质的制备新方法.系统总结了制备含有新型赖氨酸翻译后修饰蛋白质的最新化学生物学方法,包括生物正交反应策略、非天然氨基酸引入策略、"非天然氨基酸修饰法"策略等,并讨论其优缺点及应用前景.The study of native proteins with post-translational modifications（PTMs） is one of the main fields of epigenetics. The discovery of novel PTM models and their vital regulatory role for chromatin structure and gene transcription have been one of the current research focuses drawing attention of biologists especially in recent years. However, we still lack efficient strategies for the preparation of sufficient amount of native proteins with certain PTMs. The currently existing chemical biology methods are reviewed, and their advantages and disadvantages are compared, including bioorthogonal reaction technique, non-canonical amino acid incorporation, etc. Furthermore, the draft will mainly focus on the application of bioorthogonal reactions on unnatural functional groups for the incorporation of lysine PTMs.

关 键 词：蛋白质化学合成 翻译后修饰 非天然氨基酸 生物正交反应 蛋白质组学 

分 类 号：Q75[生物学—分子生物学]