解毒化瘀颗粒对急性肝衰竭大鼠肝组织TLR2、TLR4和TNF-α mRNA表达的影响  

Effect of Jiedu Huayu Granules on Expressions of TLR2,TLR4 and TNF-α mRNA on Hepatic Tissue of Acute Liver Failure Rats

作　　者：陈月桥 王挺帅 石清兰 吕建林 周玲瑶 毛德文 CHEN Yueqiao;WANG Tingshuai;SHI Qinglan;LYU Jianlin;ZHOU Lingyao;MAO Dewen(The First Affiliated Hospital of Guangxi University of TCM,Nanjing 530023,Guangxi,China;Guangxi Univeristy of TCM,Nanjing 510023,Guangxi,China)

机构地区：[1]广西中医药大学第一附属医院

出　　处：《辽宁中医杂志》2018年第9期1811-1814,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金课题(81603550,81260534,81460718);国家中医药管理局重点研究室项目;国家“十二五”科技重大专项(2012ZX10005004-003);广西自然科学基金青年基金(2013GXNSFBA019138)

摘　　要：目的：探讨解毒化瘀颗粒对急性肝衰竭（ALF）大鼠Toll样受体2（TLR2）、Toll样受体4（TLR4）及细胞因子肿瘤坏死因子α（TNF-α）mRNA表达的影响及其拮抗肝衰竭的机制。方法：将120只Wistar大鼠随机分为空白对照组、模型对照组、中药组和阳性对照组,各30只。一次性腹腔注射D-氨基半乳糖（D-Gal）/脂多糖（LPS）构建急性肝衰竭大鼠模型。观察各组大鼠存活率,检测各组大鼠血清ALT、AST水平,RT-PCR法检测肝组织中TLR2、TLR4和TNF-αmRNA的表达。结果：（1）与模型对照组比较,中药组及阳性对照组均能提高肝衰竭大鼠48 h的存活率（均P〈0.05）,虽然中药组48 h存活率高于阳性对照组,但差异无统计学意义（P=0.464）;（2）模型对照组大鼠血清AST、ALT水平均高于空白对照组（均P=0.000）,中药组和阳性对照组均低于模型对照组（均P〈0.000）,虽中药组与阳性对照组AST、ALT比较差异无统计学意义（均P〉0.05）;（3）RT-PCR结果显示,中药组和阳性对照组TLR2、TLR4、TNF-αmRNA表达较模型对照组明显降低（均P=0.000）,与阳性对照组比较,中药组TLR2、TLR4、TNF-αmRNA表达差异无统计学意义（均P〉0.05）。结论：解毒化瘀颗粒可通过降低TLR2、TLR4、TNF-αmRNA的表达,从而降低促炎因子的生成、提高肝衰竭大鼠存活率和拮抗肝衰竭。Objective： In order to explore the effect of Jiedu Huayu Granule on the expressions of toll-like receptors 2（TLR2）,toll-like receptors 4（TLR4） and tumor necrosis factor alpha（TNF-α） and the mechanism of antagonizing liver failure. Methods： A total of 120 Wistar rats were randomly divided into blank control group,model control group,traditional Chinese medicine（TCM） group and positive control group,30 rats in each group. D-galactosamine（D-Gal,800 mg/kg） and lipopolysaccharide（LPS,10 g/kg） were administered intraperitoneally into the rats. We observed rats＇ survival situation after 48 hours.And then we detected the levels of ALT and AST. The method of RT-PCR was used to detect the expressions of TLR2,TLR4 and TNF-α mRNA. Results：（1）Compared with the model group,the TCM group and the positive control group could improve the survival rate of 48 hours in liver failure rats（P = 0. 004,0. 006）. There was no significant difference between the two groups（P =0. 464）.（2）Compared with the model control group,the levels of AST and ALT were more lower in TCM group and positive control group,and the difference was statistically significant（P = 0. 000. But the difference had no statistical significance betweens two groups（all P〉0. 05）.（3）Compared with the model control group,the expressions of TLR2,TLR4 and TNF-α mRNA were decreased significantly（all P = 0. 000）. But the difference had no statistical significance between two group（all P〉0. 05）. Conclusion： Jiedu Huayu Granules can reduce the generation of pro-inflammatory cytokines,improve survival rates and antagonize liver failure through reducing the expressions of TLR2,TLR4 and TNF-α mRNA.

关 键 词：解毒化瘀颗粒 急性肝衰竭 TLR2 MRNA TLR4mRNA TNF-Α MRNA 

分 类 号：R285[医药卫生—中药学]