蒙药蓝盆花UHPLC特征图谱研究  

Study on the UHPLC Specific Chromatograms of Traditional Mongolian Medicine Flos Scabiosae

作　　者：刘艳红[1] 欧阳辉[2] 黄小方[2] 黎田儿 周茂福[2] LIU Yan-hong;OUYANG Hui;HUANG Xiao-fang;LI Tian-er;ZHOU Mao-fu(The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China;Jiangxi University of Traditional Chinese Medicine,Nanehang 330004,China)

机构地区：[1]江西中医药大学附属医院,南昌330006 [2]江西中医药大学,南昌330004

出　　处：《江西中医药大学学报》2018年第6期72-74,共3页Journal of Jiangxi University of Chinese Medicine

基　　金：江西民族传统药现代科技与产业发展协同创新中心开放项目(JXXT201402009)

摘　　要：目的:建立蓝盆花药材的UHPLC特征图谱并运用LC-MS技术对主要色谱峰进行定性分析。方法:采用Welch Ultimate UHPLC XB-C18(2. 1mm×100 mm,1. 7μm)色谱柱;以0. 3%磷酸水-乙腈为流动相梯度洗脱;流速为0. 25mL·min-1;柱温40℃;检测波长254nm;进样量3μL。结果:首次建立了蓝盆花药材的UHPLC特征图谱共有模式,共检定出22个相对保留时间稳定的共有峰; 10个特征峰初步鉴定为:芹菜素、木犀草苷、大波斯菊苷、野漆树苷、木犀草素、异荭草苷、咖啡酸、绿原酸、芦丁、槲皮素。各样品特征图谱与对照特征图谱的相似度均大于0. 99。结论:该方法准确、可靠,可为蓝盆花药材质量控制提供科学的方法。To study the HPLC specific chromatogranls testing method of the Flos Scabiosae. and to identify the primary chromatogranl peaks by LC - MS. Methods ： UHPLC was utilizaed for quality assessment of 10 batches of samples. UHPLC analysis was performed on Welch Ultimate UHPLC C18 column （100mm × 2. 1mm,1.7μm） with the mixture of acetonitrile （A） and 0.3% phosphonic acid solution（B） as mobile phase in gradient mode. The flow rate was 0.25 mL · min ^-1 The detective UV wavelength was 245 nm. Results： Twenty- - two common peaks from the specific ehromatograms of 10 batches of Flos Seabiosae were determined, 10 peaks were identified by LC - MS. The similarities of the specific ehromatograms of 10 batches of samples were all above 0.99. Conclusion： This method is accurate and reliability, which could offer scientific approach for quality control of Flos Scabiosae.

关 键 词：蒙药蓝盆花 UHPLC 特征图谱 

分 类 号：R284[医药卫生—中药学]