银屑病患者皮损间充质干细胞环状RNA表达异常研究  

Aberrant expression of circular RNA in mesenchymal stem cells from psoriatic skin lesions

作　　者：刘瑞风[1] 杨晓红[1] 梁见楠 张开明[1] Liu Ruifeng,Yang Xiaohong,Liang Jiannan,Zhang Kaiming(Department of Dermatology, Taiyuan Central Hospital, Taiyuan 030009, China)

机构地区：[1]太原市中心医院皮肤科,030009

出　　处：《中华皮肤科杂志》2018年第10期723-728,共6页Chinese Journal of Dermatology

基　　金：国家自然科学基金（81271768、81472888、81773336）

摘　　要：目的 探讨环状RNA（circRNA）在银屑病发病中的作用。方法 分离、培养15例银屑病患者皮损及15例健康对照皮肤间充质干细胞（MSC），用流式细胞仪及多向分化法进行鉴定。用RNA测序法检测circRNA表达，并进行详细的生物信息学分析。挑选7个差异表达的circRNA构建circRNA-microRNA相互作用网络，从中挑选3个与其相关的microRNA进行qRT-PCR验证。银屑病患者组和对照组qRT-PCR验证结果采用两独立样本t检验。结果 RNA测序结果显示，共检测到6 323个circRNA，其中3 227个为本实验首次发现。与对照组相比，患者组中有129个circRNA呈差异表达，其中123个表达上调，6个表达下调。挑选7个差异circRNA进行circRNA-microRNA相互作用预测，显示与这7个circRNA关系密切的银屑病相关microRNA，包括miR-17-5p、miR-30e-5p、miR-142-3p/5p、miR-369-3p、miR-184、miR-4490、miR-654-3p、miR-423-5p等。qRT-PCR也证实，这7个差异circRNA在患者组也表达上调。与对照组相比，挑选的3个microRNA在银屑病皮损中低表达（t值分别为3．993、3．217、2．918，均P〈0．05）。结论 银屑病皮损MSC circRNA表达异常，并可能参与了银屑病发病。Objective To investigate the role of circular RNA （circRNA） in the occurrence of psoriasis. Methods Mesenchymal stem cells were isolated from skin lesions of 15 patients with psoriasis （psoriasis group） and skin tissues of 15 healthy human controls （control group） separately, and then subjected to cultivation. Flow cytometry and cell differentiation assay were performed to identify these mesenchymal stem cells. RNA sequencing was conducted to measure the expression of circRNA, and detailed bioinformatics analysis was performed. Then, 7 differentially expressed eircRNAs were selected, and a circRNA-microRNA interaction network was established. In this network, 3 related microRNAs were selected and validated by real- time quantitative reverse transcription- polymerase chain reaction （qRT-PCR）. Statistical analysis was carried out by a two-sample t-test for comparison of qRT-PCR results between the psoriasis group and control group. Results As RNA sequencing showed, a total of 6 323 circRNAs were identified, and 3 227 out of these circRNAs were first reported in this study. Compared with the control group, 129 cireRNAs were differentially expressed in the psoriasis group, including 123 upregulated eireRNAs and 6 down-regulated circRNAs. The predicted circRNA-microRNA interaction network showed that several psoriasis-related microRNAs were associated with the 7 circRNAs, such as miR-17-5p, miR-30e-5p, miR-142-3p/5p, miR-369-3p, miR-184, miR-4490, miR-654-3p and miR-423-5p. qRT-PCR also confirmed that the 7 differentially expressed circRNAs were up-regulated in the psoriasis group. Compared with the control group, the expression of the 3 selected microRNAs significantly decreased in the psoriasis group （t = 3.993, 3.217, 2.918, respectively, all P 〈 0.05）. Conclusion There is aberrant expression of circular RNAs in the mesenchymal stem ceils from psoriatic skin lesions, which may take part in the occurrence of osoriasis.

关 键 词：银屑病 间质干细胞 环状RNA 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]