嗅三针对阿尔茨海默病小鼠学习能力和海马IL1-β、IL-6表达的影响  

Influence of “Xiusanzhen” Electroacupuncture on Learning Ability and IL1-β,IL-6 in Hippocamus for Mice with Alzheimer's Disease

作　　者：王渊[1,2] 刘智斌 牛文民[1,2] 王强 WANG Yuan, LIU Zhibin, NIU Wenmin, et al.(1. Shanaxi University of Traditional Chinese Medicine （ Xianyang Shannxi 712046, China） ; 2. Shanxi Key Laboratory of Acupuncture and Medicine （ Xianyang Shanxi 712046, China）)

机构地区：[1]陕西中医药大学,陕西咸阳712046 [2]陕西省针药结合重点实验室,陕西咸阳712046

出　　处：《四川中医》2018年第9期33-36,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：国家自然科学基金(编号:81503667);陕西中医药大学重点培育科研基金(编号:2015PY07)

摘　　要：目的:观察"嗅三针"对阿尔茨海默病(AD)小鼠学习和海马IL1-β和IL-6表达的影响,以阐明其基于嗅觉通路治疗AD的作用机制。方法:采用APP/PS1转基因小鼠作为AD小鼠模型,小鼠嗅觉通路阻断模型采用嗅神经切断术建立,将40只AD小鼠随机分为模型对照组、嗅神经切断嗅三针组、嗅三针组、盐酸多奈哌齐组,每组10只,另取10只同窝的阴性小鼠作为正常对照组。通过"嗅三针"对印堂穴和双侧迎香穴行电针刺激,每次10分钟,以3mg/kg/d的剂量对盐酸多奈哌齐组AD小鼠进行灌胃,每周干预5次,间歇2天,共干预4周。小鼠学习能力采用Morris水迷宫进行监测,应用Western blot技术检测海马IL1-β和IL-6表达水平。结果:嗅三针刺激和盐酸多奈哌齐灌胃能够显著缩短AD小鼠平均逃避潜伏期,并能显著降低海马IL1-β和IL-6的表达,与AD模型组比较,差异具有显著性(P<0. 05);嗅神经切断嗅三针组对AD小鼠平均逃避潜伏期以及海马IL1-β和IL-6的表达均无明显影响,与AD模型组比较,差异无显著性(P>0. 05),嗅三针组与盐酸多奈哌齐组比较无显著性差异(P>0. 05)。结论:"嗅三针"具有改善AD小鼠学习能力,降低海马水平IL1-β和IL-6表达的作用,嗅觉通路的完整性可能是其发挥干预效应的核心机制。Objecticve： To observe the intervening effect of＂Xiusanzhen＂Electroacupuncture（ EA） on learning and memory ability and IL1-β,IL-6 expression in hippocampus of mice with Alzheimer＇s disease（ AD）,in order to clarify its therapeutic mechanism based on the olfactory nerve pathway in alleviating AD. Methods： APP/PS1 transgenic mice were used as AD mice models,mice model with olfactory nerve pathway block were established by transecting olfactory nerve,40 AD mice were randomly devided into 4 groups： AD model group,＂Xiusanzhen＂EA with olfactory nerve transected group（ ONT＋EA）,＂Xiusanzhen＂EA group,Donepezil HCl group,with 10 mice respectively in each group,then took 10 negative mice as normal controlled group.Electroacupuncture intervention of＂Xiusanzhen＂was applied to stimulate bilateral LI 20 and EX-HN3 for 10 min per time,intragastric administration of donepezil HCI in AD mice was performed at the dose of 3 mg/kg/d,once daily intervention（ except weekends） for 4 weeks in all. Morris water maze was applied to test learning ability of mice. RT-PCR and agarose gel electrophoresis were applied to test IL1-β and IL-6 expression in hippocampus of mice. Results： Compared with AD model group, ＂Xiusanzhen＂EA group Donepezil HCl group could shorter mean escape latency,meanwhile,reduce IL1-β and IL-6 expression in hippocampus of AD mice,there were significant significant differences between the two groups（ P〈0. 05）; There were no significant differences were found between AD model and ONT ＋ EA groups in influencing mean escape latency and IL1-β,IL-6 expression in hippocampus of AD mice（ P〉0. 05）,there were no significant difference between ＂Xiusanzhen＂EA group and donepezil HCl group（ P〉0. 05）. Conclusion： ＂Xiusanzhen＂EA has the effect in ameliorating learning ability and reducing the IL1-β and IL-6 expression in hippocampus of mice with AD,the integrity of the olfactory pathway is the core mechanism of the intervention effect.

关 键 词：阿尔茨海默病 嗅三针 学习能力 海马 

分 类 号：R245.3[医药卫生—针灸推拿学] R742.5[医药卫生—中医临床基础]