辽宁绒山羊副结核分枝杆菌的分离鉴定及分子生物学检测  

Isolation,Identification and Molecular Biological Detection of Mycobacterium Tuberculosis from Liaoning Cashmere Goats

作　　者：宋先忱[1] 李冰 朱延旭[1] 张兴会[1] 王世泉[1] 郭伶 徐鹏 郭维军[3] 刘孝刚 SONG Xian-chen;LI Bing;ZHU Yan-xu;ZHANG Xing-hui;WANG Shi-quan;GUO Ling;XU Peng;GUO Wei-jun;LIU Xiao-gang(Liaoning Animal Science Research Institute,Liaoyang 111000,China;College of Animal Science and Veterinary Medicine,Jinzhou Medical University,Jinzhou 121001,China;Jinzhou Center for Animal Disease Controland Prevention,Jinzhou 121002,China)

机构地区：[1]辽宁省畜牧科学研究院,辽宁辽阳111000 [2]锦州医科大学畜牧兽医学院,辽宁锦州121001 [3]锦州市动物疫病预防控制中心,辽宁锦州121002

出　　处：《中国兽医杂志》2018年第6期32-34,I0001,共4页Chinese Journal of Veterinary Medicine

基　　金：辽宁省农业攻关及产业化项目:舍饲绒山羊营养代谢病及副结核病防控技术(2015103036)

摘　　要：为了对某辽宁绒山羊饲养场疑似副结核病例作出正确诊断,本研究针对疑似病羊的内脏组织和肠内容物在Dubods油酸琼脂培养基上经过37℃条件下9～13周培养,从其肝脏组织中分离获得1株细菌,对该分离菌株开展了形态学观察、抗酸染色镜检、PCR检测及基因序列测定等研究工作。结果显示,分离菌为抗酸染色阳性菌,而且形态学符合副结核分支杆菌的特征; PCR检测结果为阳性,测定的序列与Gen Bank中JⅡ-1961(CP022105. 1)基因序列同源性高达100%,证实了该分离菌株为副结核分支杆菌。In order to make a correct diagnosis of suspected tuberculosis cases in one Liaoning cashmere goat farm, we conducted the study on the Dubods oleic acid agar medium for 9-13 weeks at 37 ℃ for the visceral tissue and intestinal contents of suspected sheep, A strain was isolated from its liver tissue. Morphological observation, acid-resistant staining, PCR detection and gene sequen- cing were carried out. The results showed that the isolates were positive for acid-fast staining and the morphology was consistent with the characteristics of Mycobacterium tuberculosis. The results of PCR were positive and the sequence was 100% homologous to J II - 1961 （CP022105. 1 ） gene sequence in GenBank, Confirming that the isolate was Mycobacterium tuberculosis.

关 键 词：副结核病 病原分离鉴定 分子生物学检测 辽宁绒山羊 

分 类 号：S855.12[农业科学—临床兽医学]