奶牛乳腺炎金黄色葡萄球菌FnBPA蛋白免疫研究  

Immunity Study on FnBPA Protein of Staphylococcus aureus from Cow Mastitis

作　　者：马保臣 董玉兰[3] 柴同杰[2] MA Bao-chen;DONG Yu lan;CHAI Tong-jie(China Animal Husbandry Grdup,Beijing 10070,China;College of Animal science and Technoloy,Tai＇an 271018,China;College of Veterinay Medicine,China Agicultuml University,Beijing 100193,China)

机构地区：[1]中国牧工商集团有限公司,北京丰台100070 [2]山东农业大学动物科技学院,山东泰安271018 [3]中国农业大学动物医学院,北京海淀100193

出　　处：《中国兽医杂志》2018年第6期49-52,共4页Chinese Journal of Veterinary Medicine

基　　金：德国政府基金会(DAAD)国际优秀专家学者资助项目(423-China;Mongolei)

摘　　要：为了研究奶牛乳腺炎金黄色葡萄球菌发病机制和治疗措施,对引起奶牛临床型和隐性乳腺炎的337株金黄色葡萄球菌进行FnBPA蛋白基因分子调查,并且表达和纯化了FnBPA蛋白,制作FnBPA蛋白疫苗进行动物免疫试验。结果表明,FnBPA蛋白基因携带率为51. 04%,临床型乳腺炎金黄色葡萄球菌FnBPA蛋白基因的携带率为68. 5%。选取分离菌株10A扩增出的FnBPA基因,进行原核表达,重组蛋白得到高效表达,获得高纯度的FnBPA蛋白,BCA法蛋白定量为2. 8 mg/mL,制作蛋白疫苗免疫3头试验奶牛,30～40 d后,ELISA检测血清抗体滴度达到高峰,最高抗体滴度达到7l og_2,该蛋白具有很好的抗原性。In order to study the pathogenesis and treatment of Staphylococcus aureus in cow mastiffs, FnBPA gene from 337 strains of Staphylococcus aureus, which cause cliv.ical and subclinical mastitis were investigated. Then, FnBPA protein was expressed and puri- fied ,and Immunization Test of FnBPA protein vaccine was conducted in a farm. The results showed that the rate of FnBPA was 51.04% and the rate of FnBPA of Staphyloax.cus aureus in clinical mastitis was 68.5%. The strain IOA FnBPA gene was expressed and the re- combinant protein was efficiently expressed. The high purity of FnBPA protein was obtained in this experiment, and the protein content was 2.8 mg/mL, 3 cows were immunized with the protein vaccine, and the serum antibody reached the its peak after 30 -40 day, and the highest antibody titer reached 7log2 measured by ELISA. The protein showed good antigenicity.

关 键 词：奶牛 金黄色葡萄球菌 FnBPA蛋白 

分 类 号：S858.23[农业科学—临床兽医学]