盐胁迫下耐盐番茄根的蛋白质组分析  

Proteomic analysis of root of salt–tolerant tomato under salt stress

作　　者：钟克焱 顾骁 马启林 邓用川 夏幽泉 ZHONG Keyan;GU Xlao;MA Qlhn;DENG Yongchuan;XIA Youquan(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学热带农林学院,海南海口570228

出　　处：《湖南农业大学学报（自然科学版）》2018年第5期495-499,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(31260354;31660381)

摘　　要：以导入红海榄总DNA的耐盐番茄及其野生型番茄为材料,于3~4片真叶期分别用调和海水(1.07%盐度)和自来水进行灌溉,处理14d后,采用BPP法提取番茄根部的全蛋白质,通过等电聚焦和SDS–PAGE电泳得到番茄根部全蛋白质双向电泳图谱。利用Image Master 2D Platinum 6.0对双向电泳图谱进行分析发现,供试番茄根部全蛋白质双向电泳图谱大约有550个蛋白质点,其中耐盐番茄与野生型番茄蛋白质表达量比值大于1.5或小于0.66的有139个;海水处理下,耐盐番茄与野生型番茄根部蛋白质表达量比值大于2.0或小于0.5的有69个,其中37个蛋白点的表达量为上调,28个蛋白点的表达量为下调,此外还有4个特异蛋白点。The salt–tolerant tomato introducd total DNA of Rhizophora stylosa griff and its wild type tomato was used as materials, which was irrigated respectively with blended sea water （mixed with seawater and freshwater according to 1：2, 1.07% salinity） and freshwater for 14 days during three to four true leaf stage. Total root protein of tomato was extracted with BPP method, and the proteomic profiles of roots of salt–tolerant tomato and wild types under different treatments was gotten by IEF and subsequent SDS–PAGE. The differential analysis of two–dimensional gel electrophoresis profiles were analyzed using Image Master 2D platinum 6.0 software. The results showed about 550 protein pots were found in the proteomic profiles of two–dimensional gel electrophoresis and 139 differential protein pots which protein expression ratios greater than 1.5 or less than 0.66 were detected. Sixty nine differential protein spots which protein expression ratios greater than 2.0 or less than 0.5, including 37 up–regulated ones, 28 down–regulated ones and 4 uniqueones, were detected in root protein profiles between salt–tolerant tomato and its wild type under the condition of salt water irrigation.

关 键 词：番茄 耐盐性 双向电泳 差异蛋白 

分 类 号：S641.201[农业科学—蔬菜学]