ARB类药物对2K1C型肾血管性高血压大鼠左室重构方面的类效应及作用研究  

Class Effect of ARBs on the Left Ventricular Remodeling in 2K1C Renovascular Hypertensive Rats

作　　者：徐庆波 白广海 许晓茹 王恒亮 Xu Qingbo;Bai Guanghai;Xu Xiaoru;Wang Hengliang(Puyang Oilfield General Hospital,Xinxiang Medical University,Puyang 457001,Henan,China)

机构地区：[1]新乡医学院附属濮阳市油田总医院,河南濮阳457001

出　　处：《中西医结合心脑血管病杂志》2018年第18期2616-2620,共5页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

摘　　要：目的以氯沙坦、缬沙坦、替米沙坦、厄贝沙坦4种血管紧张素Ⅱ受体阻滞剂(ARB)类药物对二肾一夹(2K1C)型肾血管性高血压大鼠进行干预,观察其血压、心脏指数、左心室指数、Ⅰ型胶原、Ⅲ型胶原及核因子-κB(NF-κB)的改变,研究4种ARB类药物在改善2K1C型肾血管性高血压大鼠左室重构方面的类效应,并初步探讨NF-κB在2K1C型肾血管性高血压大鼠左室重构中的作用。方法将56只雄性SD大鼠随机分为空白对照组(n=8)、假手术组(n=8)以及模型组(n=40),模型以改良的二肾一夹方法建立。模型建立成功后,模型组随机分为4个治疗组及模型对照组(n=8),治疗组按照药品说明书对临床使用的推荐剂量分别给予氯沙坦(30 mg/kg)、缬沙坦(30 mg/kg)、替米沙坦(30 mg/kg)、厄贝沙坦(50 mg/kg)灌胃;假手术组、空白对照组及模型对照组分别给予相同体积的生理盐水灌胃,每日1次。比较各组血压、心脏指数、左心室指数、Ⅰ型胶原、Ⅲ型胶原含量的改变以及NF-κB的表达。结果模型对照组与空白对照组比较,心脏指数及左心室指数均增高,差异有统计学意义(P <0.05)。各治疗组与模型对照组比较,用药8周血压、心脏指数、左心室指数均降低,差异有统计学意义(P <0.05)。假手术组与空白对照组比较,NF-κB、Ⅰ型胶原、Ⅲ型胶原差异无统计学意义(P>0.05)。模型对照组与空白对照组比较,NF-κB、Ⅰ型胶原、Ⅲ型胶原的表达明显增高,差异有统计学意义(P <0.05)。4个治疗组与模型对照组比较,NF-κB、Ⅰ型胶原、Ⅲ型胶原的表达明显减少,差异有统计学意义(P <0.05)。氯沙坦组NF-κB的表达高于厄贝沙坦组、替米沙坦组以及缬沙坦组,各治疗组Ⅰ型胶原、Ⅲ型胶原的表达比较差异无统计学意义(P>0.05)。结论 NF-κB参与了高血压大鼠左室重构的病理过程。4种ARB类药物均可通过抑制NF-κB的表达来改善2K1C型高血压大鼠左室�Objective To investigate the effect of angiotensin Ⅱ receptor blockers （ARBs） such as losartan,valsartan,telmisartan and irbesartan on blood pressure （BP）,cardiac index,left ventricular index,type I collagen,type Ⅲ collagen and the expression of nuclear factor κB （NF κB） in 2 kidney,one clip （2K1C） renovascular hypertensive rats,and investigate the role of NF κB in left ventricular remodeling in 2K1C renovascular hypertensive rats. Methods Fifty six male Sprague Dawley （SD） rats were randomly devided into three groups：blank control group （ n =8）,sham operation group （ n =8）,and model group （ n =40）.The rat model were established by 2K1C.After the model was successfully established,the rats in model group were randomly divided into losartan group,valsartan group,telmisartan group,irbesartan group,and model control group,8 rats in each group.The rats were administrated by normal saline for gavage in blank control group,sham operation group,and model control group.The rats were administrated by losartan （30 mg/kg）,valsartan （30 mg/kg）,telmisartan （30 mg/kg）,and irbesartan （50 mg/kg） for gavage in losartan group,valsartan group,telmisartan group,and irbesartan group,respectively.After eight weeks of administration,blood pressure,heart index,left ventricular index,type Ⅰ collagen,type Ⅲ collagen,and NF κB expression were observed. Results Compared with the blank control group,the cardiac index,left ventricular index increased in model control group （ P 〈0.05）.Compared with the model control group,blood pressure,cardiac index and left ventricular index were decreased in losartan group,valsartan group,telmisartan group,and irbesartan group at 8 weeks of administration （ P 〈0.05）.There was no significant difference in the expressions of NF κB ,type Ⅰ collagen and type Ⅲ collagen between the sham operation group and the blank control group （ P 〉0.05）.Compared with the blank control group,the expressions of NF κB,type Ⅰ collagen and

关 键 词：肾血管性高血压 二肾一夹型 血管紧张素Ⅱ受体阻滞剂 核因子-ΚB 左室重构 类效应 

分 类 号：R544.1[医药卫生—心血管疾病] R255.3[医药卫生—内科学]