戒毒瘾丸对大鼠吗啡戒断症状的影响  

Effects of Jieduyin Pills on morphine withdrawal symptoms in rats

作　　者：陈玉兴 李茹月 李钰婷 黄雪君 曾晓会 黄丹娥 甘海宁 CHEN Yu-xing;LI Ru-yue;LI Yu-ting;HUANG Xue-jun;ZENG Xiao-hui;HUANG Dan-e;GAN Hai-ning(Guangdong Provincial Research Institute for Traditional Chinese Medicine Engineering Technology,Guangzhou 510095,China;Guangdong Provincial Key Laboratory for Research and Development of Traditional Chinese Medicine,Guangzhou 510095,China;Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广东省中医药工程技术研究院,广东广州510095 [2]广东省中医药研究开发重点实验室,广东广州510095 [3]广州中医药大学,广东广州510405

出　　处：《中成药》2018年第10期2126-2130,共5页Chinese Traditional Patent Medicine

基　　金：广东省科技计划项目(2013B040200040;2017A070701017)

摘　　要：目的观察戒毒瘾丸对大鼠吗啡戒断症状的影响。方法 70只大鼠随机分为正常对照组、模型对照组、可乐定片组(0. 08 mg/kg)、济泰片组(0. 43 g/kg)及戒毒瘾丸高、中、低剂量组(7. 92、3. 96、1. 98 g生药/kg),每组10只,除正常对照组外各组建立吗啡依赖大鼠的自然戒断模型;另取90只大鼠随机分为正常对照组(10只)、吗啡依赖模型组(80只),其中后一组于第10天再分为模型对照组、可乐定片组、济泰片组及戒毒瘾丸高、中、低剂量组(给药剂量同上),每组10只(剔除评分过高或过低、状态不理想的20只大鼠),建立吗啡依赖大鼠的纳洛酮催促戒断模型,记录给药后戒断评分,测定前一模型血清5-HT、DA含有量及后一模型额叶皮质NE、5-HT、内啡肽含有量。结果与模型对照组比较,戒毒瘾丸组戒断评分及5-HT、DA、NE、5-HT含有量显著下降(P <0. 01),内啡肽含有量显著升高(P <0. 01)。结论戒毒瘾丸可通过降低5-HT、DA、NE含有量、升高内啡肽含有量来减轻大鼠吗啡戒断症状。AIM To observe the effects of Jieduyin Pills on morphine withdrawal symptoms in rats. METHODS Seventy rats randomly divided into normal control group, model control group, Clonidine Tablets group （0.08 mg/kg）, Jitai Tablets group （0.43 g/kg） and high-dose, medium-dose, low-dose Jieduyin Pills groups （7.92, 3.96, 1.98 g crude drug/kg） , ten rats in each group, were cultivated as the natural withdrawal models for morphine-dependent rats, except for those of the normal control group, and the models had their post- administration withdrawal scores recorded, serum 5-HT and DA contents determined. Another 90 rats were random- ly divided into normal control group （10 rats） and morphine-dependent group （80 rats）. The 80 rats were subse- quently assigned into model control group, Clonidine Tablets group, Jitai Tablets group and high-dose, medium- dose, low-dose Jieduyin Pills groups, ten rats in each group （20 rats were eliminated due to reasons of their poor scolds or unsatisfactory performance） , dosed with the medical agent and noloxine to build opioid-naloxone with- drawal rat models and finally had their post-administration check of frontal cortex NE, 5-HT, endorephin contents. RESULTS Compared with the model control group, Jieduyin Pills groups shared significantly lower withdrawal scores, less 5-HT, DA, NE, 5-HT contents （P 〈 0.01 ）, and marked increase of endorphin content （P 〈 0.01 ）. CONCLUSION Jieduyin Pills can alleviate morphine withdrawal symptoms in rats by decreasing 5-HT, DA, NE contents and increasing endorphin content.

关 键 词：戒毒瘾丸 吗啡 戒断症状 

分 类 号：R285.5[医药卫生—中药学]