高效液相色谱法测定复方银杏通脉口服液中总黄酮醇苷含量  

Content Determination of Total Flavonol Glycoside in Compound Yinxing Tongmai Oral Liquid by HPLC

作　　者：张耕[1] 刘译[1] 唐勇[1] 杨跃龙[1] Zhang Geng;Liu Yi;Tang Yong;Yang Yuelong(Huaihua Institute for Food and Drug Control,Huaihua,Hunan,China 418000)

机构地区：[1]湖南省怀化市食品药品检验所,湖南怀化418000

出　　处：《中国药业》2018年第20期12-15,共4页China Pharmaceuticals

基　　金：湖南省食品药品监督管理局食品药品安全科技项目[湘食药科R201725]

摘　　要：目的建立测定复方银杏通脉口服液中总黄酮醇苷含量的高效液相色谱(HPLC)法。方法色谱柱为Shimadzu C_(18)柱(250 mm×4. 6 mm,5μm),流动相为甲醇-0. 4%磷酸溶液(55∶45),流速为1. 0 m L/min,柱温为25℃,进样量为10μL,检测波长为360 nm,外标法定量。结果成功建立回归方程,槲皮素为Y=40 994 X-15 542,r=0. 999 8(n=7),线性范围为0. 014 8~0. 148 6μg;山柰素为Y=42 969 X-11 186,r=0. 999 8(n=7),线性范围为0. 014 9~0. 149 6μg;异鼠李素为Y=37 968 X-9 333. 6,r=0. 999 7(n=7),线性范围为0. 007 9~0. 079 5μg。平均加样回收率,槲皮素为97. 30%,RSD=1. 61%(n=6);山柰素为97. 50%,RSD=1. 44%(n=6);异鼠李素为97. 83%,RSD=1. 63%(n=6)。结论该方法操作简单,结果准确,重复性好,可用于复方银杏通脉口服液的质量控制。Objective To establish an HPLC method for content determination of total flavonol glycoside in Compound Yinxing Tongmai Oral Liquid. Methods The chromatographic column was Shimadzu C_（18） column（ 250 mm × 4. 6 mm,5 μm）,the mobile phase was methanol-0. 4% phosphoric acid solution（ 55 ∶ 45）,the flow rate was 1. 0 m L/min,the column temperature was 25 ℃,the sample size was 10 μL,and the detection wavelength was 360 nm. Quantification was performed by the external standard method. Results The regression equation was successfully established： the quercetin was Y = 40 994 X-15 542,r = 0. 999 8（ n = 7）,it had a good linear relation in the range of 0. 014 8-0. 148 6 μg. The kaempferol was Y = 42 969 X-11 186,r = 0. 999 8（ n = 7）,it had a good linear relation in the range of 0. 014 9-0. 149 6 μg. The isorhamnetin was Y = 37 968 X-9 333. 6,r = 0. 999 7（ n = 7）,it had a good linear relation in the range of 0. 007 9-0. 079 5 μg. The average recoveries of quercetin,kaempferol and isorhamnetin were 97. 30%（ RSD = 1. 61%,n = 6）,97. 50%（ RSD = 1. 44%,n = 6）,97. 83%（ RSD = 1. 63%,n = 6）. Conclusion The method is simple,accurate and reproducible,which can be used for the quality control of Compound Yinxing Tongmai Oral Liquid.

关 键 词：复方银杏通脉口服液 总黄酮醇苷 高效液相色谱法 质量控制 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学]