超高效液相色谱法对丹参不同部位化学成分的研究  

Analysis of chemical constituents of different parts in Salvia Miltiorrhiza by UPLC

作　　者：陈旭青[1] 陈醒[1] 吴苏亚 骆雨璇[1] 王楠[1] CHEN Xuqing;CHEN Xing;WU Suya;LUO Yuxuan;WANG Nan(Department of Pharm,Nanjing General Hospital,Nanjng Military Area Commarut,Nanjing,Jiangsu 210002,China)

机构地区：[1]南京军区南京总医院药品科,江苏南京210002

出　　处：《安徽医药》2018年第11期2096-2099,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的对丹参(叶、茎、花、根)不同部位中的可利用化学成分进行含量测定与分析。方法采用超高效液相色谱——二极管阵列检测器(UPLC-PDA)法对丹参(叶、茎、花、根)中酚酸类、黄酮类成分的含量进行分析评价。结果丹参中不同部位中丹酚酸类、黄酮类含量差异较大,丹参地上部分(叶、茎、花)中除了含有大量丹酚酸类成分外,相对根而言,还富含以芦丁为主的黄酮类成分,叶、茎、花中分别含有芦丁的质量分数为3.53、0.56、0.66 mg·g^(-1)。结论丹参叶、茎、花中含有大量的丹参酚酸类、黄酮类等资源性化学成分,对丹参资源利用效率提升以及扩大丹参新药源具有重要科学意义和开发前景。Objective To determine and analyze the available chemical contents in different parts（ leaves,stems,flowers,roots） of Salvia Miltiorrhiza. Methods The phenolic acids and flavonoids in different parts of Salvia Miltiorrhiza were analyzed and evaluated by ultra-high performance liquid chromatography-photo-diode array（ UPLC-PDA）. Results The results showed that the contents of salvianolic acids and flavonoids in different parts of Salvia Miltiorrhiza were significantly different. Compared with the roots,the aerial part（ leaves,stems,flowers） of Salvia Miltiorrhiza were rich of flavonoids which were mainly composed of rutin besides phenolic acids,and the leaves,stems,and flowers of Salvia Miltiorrhiza contained rutin at 3. 53,0. 56,0. 66 mg·g^-1,respectively. Conclusion The analysis shows that aerial part of Salvia Miltiorrhiza（ leaves,stems and flowers） contain a large amount of phenolic acids and flavonoids,which has important scientific significance and development prospects for improving the utilization efficiency and expanding the new drug sou-rce of Salvia Miltiorrhiza.

关 键 词：丹参 分析评价 丹酚酸类 黄酮类 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]