基于TK基因突变试验评价卷烟烟气的遗传毒性  

Genotoxicity of cigarette smoke by using the TK gene mutation test

作　　者：尚平平[1] 李翔[1] 谢复炜[1] 霍娇 华辰凤 SAHNG Pingping;LI Xiang;XIE Fuwei;HUO Jiao;HUA Chenfeng(Zhengzhou Tobacco Research Institute of China National Tobacco Corporation,Zhengzhou 450001,China;West China School of Public Health,Sichuan University,Chengdu 610000,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州450001 [2]四川大学华西公共卫生学院

出　　处：《公共卫生与预防医学》2018年第5期23-25,共3页Journal of Public Health and Preventive Medicine

基　　金：郑州烟草研究院院长科技发展基金(322016CA0240)

摘　　要：目的采用TK基因突变试验研究卷烟烟气的遗传毒性。方法以L5178Y tk+/-3. 7. 2C小鼠淋巴瘤细胞为研究对象,3R4F参比卷烟的烟气为受试物,卷烟烟气经DMSO萃取后,设置试验剂量组为20、40、80、100、150μg/m L,另设二甲基亚砜溶剂对照组和环磷酰胺阳性对照组,对L5178Y tk+/-3. 7. 2C小鼠淋巴瘤细胞染毒处理及表达。结果在加S9的情况下,80μg/m L的突变频率是溶剂对照组3倍以上,实验结果呈阳性。结论在此次试验条件下,卷烟烟气具有TK基因致突变作用。Objective To investigate the potential genotoxicity of cigarette smoke using the TK gene mutation test. Methods The cigarette smoke condensate （ CSC） of the reference cigarette 3R4F was extracted by DMSO. The L5178Y tk^ ＋ / -3, 7, 2C mousely mphoma cells were treated with CSC at different concentrations, 20、40、80、100 and 150 μg / mL, for 3 h. Dimethyl sulfoxide was the solvent control group, and cyclophosphamide was positive control group. Results With metabolic activation, the TK gene mutation frequency of 80 μ g / mL group was more than 3 times higher than that of the solvent control group. Conclusion The results indicatethat cigarette smoke containing toxicant inducing TK gene mutation.

关 键 词：卷烟烟气 TK基因突变试验 

分 类 号：R114[医药卫生—卫生毒理学]