贵州惠水县茶轮斑病病原菌的鉴定  

Identification of the Pathogen Causing Tea Gray Blight Disease in Huishui County, Guizhou Province

作　　者：李冬雪 赵晓珍[1,2] 王勇 练珊珊 任亚峰[1] 陈卓 LI Dongxue;ZHAO Xiaozhen;WANG Yong;LIAN Shanshan;REN Yafeng;CHEN Zhuo(Key Laboratory of Green Pesticide and Agricultural Bioengineering,Ministry of Education,Guizhou University,Guiyang,Guizhou 550025,China;Guizhou Institute of Fruit Tree Science,Guiyang,Guizhou 550006,China;Agricultural College,Guizhou University,Guiyang,Guizhou 550025,China;Tea College,Guizhou University,Guiyang,Guizhou 550025,China)

机构地区：[1]贵州大学绿色农药与农业生物工程教育部重点实验室,贵州贵阳550025 [2]贵州省果树科学研究所,贵州贵阳550006 [3]贵州大学农学院,贵州贵阳550025 [4]贵州大学茶学院,贵州贵阳550025

出　　处：《热带作物学报》2018年第9期1827-1833,共7页Chinese Journal of Tropical Crops

基　　金：国家科技支撑计划专题(No.2014BAD23B03);贵州省科技重大专项[黔科合重大专项(2012)6012号];贵州省科技厅-黔南州人民政府农业科技合作专项计划(No.2013-01)

摘　　要：贵州省惠水县茶区常发生茶轮斑病,对茶叶品质和产量影响较大。为确定该病害的病原菌,本文对病害叶片的病原菌进行分离、纯化和培养,通过柯赫氏法则进行病原菌针刺法和剪切法的致病性测定。并依据病原菌核糖体内转录间隔区、β-微管蛋白、延伸因子-1α进行多基因系统发育分析。基于病原菌多基因系统发育和形态学结果,将病原菌确定为茶假拟盘多毛孢(Pseudopestalotiopsis camelliae-sinensis)。Tea gray blight disease always takes place in the tea area of Huishui County, Guizhou Province, which severely affects the quality and yield of tea leaves. To determine the pathogen of the disease, we isolated, purified and cultivated the pathogenic fungus from the diseased tea leaves, then determined its pathogenicity following Koch＇s rule with the methods of needle punch and cut. Phylogenetic analyses against multi-locus sequences, viz. rDNA-ITS, β-tublin and EF-1α were conducted. Based on the morphology and phylogenetic analyses, the pathogen was identified as Pseudopestalotiopsis camelliae-sinensis.

关 键 词：茶树 茶轮斑病 假拟盘多毛孢属 形态特征 致病性分析 系统发育分析 

分 类 号：S432.44[农业科学—植物病理学]