SENP3在成年小鼠中枢神经系统的分布及中脑多巴胺神经元内敲除SENP3的初探  

Distribution of SENP3 in adult central nervous system and preliminary exploration of conditional depletion of SENP3 in midbrain dopaminergic neurons

作　　者：于昕 任艳华 陈佳音 易静[3] 丁玉强[1] Xin Yu;Yanhua Ren;Jiayin Chen;Jing Yi;Ding Yuqiang(Department of Anatomy and Neurobiology,School of Medicine,Tongji University,Shanghai 200092;Institute of Neuroscience,Wenzhou Medical University,School of Medicine,Wenzhou 325035;Department of Biochemistry and Molecular Cell Biology,School of Medicine,Shanghai Jiao Tong University,Shanghai 200025,China)

机构地区：[1]同济大学医学院人体解剖与神经生物学系,上海200092 [2]温州医科大学基础医学院神经科学研究所,温州325035 [3]上海交通大学基础医学院生化与分子细胞生物学系,上海200025

出　　处：《神经解剖学杂志》2018年第5期603-609,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81571332)

摘　　要：目的:探讨小泛素类修饰蛋白特异性蛋白酶3(small ubiquitin-like modifier proteins specific protease3,SENP3)在成年小鼠中枢神经系统(central nervous system,CNS)内的分布特征,以及在中脑多巴胺能神经元敲除SENP3对其发育和存活的影响。方法:利用免疫组织化学染色方法观察SENP3在成年小鼠脑中的分布及表达特征;制备在酪氨酸羟化酶(tyrosine hydroxylase,TH)神经元内选择性敲除SENP3的小鼠,观察SENP3缺失对中脑TH神经元的影响。结果:SENP3免疫阳性信号在成年小鼠CNS内广泛分布,包括嗅球、海马、丘脑、黑质、腹侧被盖区、小脑和脊髓等多个脑区。在中脑TH神经元中选择性敲除SENP3后,黑质(substantia nigra,SN)和腹侧被盖区(ventral tegmental area,VTA)的TH神经元的数量及其相关基因的表达未见明显改变。结论:SENP3全脑分布的形态学资料为研究其功能提供了线索;在正常生理条件下小鼠中脑多巴胺能神经元的发育和存活不依赖于SENP3的表达。Objective： To explore the distribution of small ubiquitin-like modifier proteins specific protease 3（ SENP3） in the central nervous system（ CNS） of adult mice,and whether conditional depletion of SENP3 in midbrain dopaminergic neurons affects its development and survival.Methods： SENP3 distribution in the adult mouse brain was examined by immunohistochemistry.Mice with conditional depletion of SENP3 in tyrosine hydroxylase（ TH） neurons was generated to observe if SENP3 deficiency affects midbrain TH neurons.Results： SENP3-immunoreactive signals were widely distributed in the CNS of adult mice,including various brain regions such as the olfactory bulb,hippocampus,thalamus,substantia nigra,ventral tegmental area,cerebellum and spinal cord.After depletion of SENP3 in midbrain TH neurons,numbers of the TH neurons and expression of its associated signature genes exhibit no significant change in the substantia nigra（ SN） and ventral tegmental area（ VTA）.Conclusion： SENP3-immunoreactive signals were widely distributed,providing morphological data for exploring SENP3 functions in the brain.It is also concluded that the development and survival of TH neurons in the midbrain do not depend on the existence of SENP3 in normal physiological conditions.

关 键 词：SENP3 免疫组织化学 条件性基因敲除 酪氨酸羟化酶 小鼠 

分 类 号：R338[医药卫生—人体生理学]