太白楤木的化学成分分离鉴定  

Isolation and Identification of Chemical Constituents from Aralia taibaiensis Cortex

作　　者：梁小飞 赵媛媛 刘小照 杨新杰[1] 范妤[1] 郭东艳[1] 宋小妹[1] 宋蓓[2,3] LIANG Xiao-fei;ZHAO Yuan-yuan;LIU Xiao-zhao;YANG Xin-jie;FAN YU;GUO Dong-yan;SONG Xiao-mei;SONG Bei(School of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China;The Second Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712046,China;College of Life Sciences,Northwest University,Xi＇an 710069,China)

机构地区：[1]陕西中医药大学药学院,陕西咸阳712046 [2]陕西中医药大学第二附属医院,陕西咸阳712046 [3]西北大学生命科学学院,西安710069

出　　处：《中国实验方剂学杂志》2018年第20期56-61,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：陕西省中药基础与新药研究重点实验室开放基金项目(2017KF02);陕西省科技厅统筹项目(2013KTCQ03-12);国家自然科学基金项目(81703925)

摘　　要：目的：对太白楤木Aralia taibaiensis根皮70%乙醇部位进行系统的化学成分研究,从物质基础的角度探寻其作为药用资源的可能性。方法：取太白楤木药材3 kg,70%乙醇浸泡提取,经减压浓缩后得到太白楤木浸膏,将浸膏加适量水分散,得到太白楤木上样液,将上样液经101大孔树脂柱色谱吸附,依次用水和70%乙醇洗脱,得到2个部位。对其70%乙醇部分通过硅胶柱色谱,Sephadex LH-20凝胶柱色谱,半制备HPLC及重结晶等方法进行分离纯化,并且利用NMR,MS等现代波谱技术以及化合物的理化性质并结合参考文献确定化合物的结构。结果：从太白楤木70%乙醇部位中分离得到12个三萜皂苷类化合物,分别鉴定为araliasaponinⅫ（1）,竹节参皂苷1（2）,竹节参皂苷Ⅳa（3）,tarasaponin V（4）,蓣知子皂苷Ⅳ（5）,elatoside F（6）,araliasaponinⅡ（7）,araliasaponinⅥ（8）,araliasaponinⅢ（9）,楤木皂苷A（10）,银莲花苷（11）,3-O-[β-Drhamnopyranosy（1→2）-α-L-arabinopyranosy]-28-O-[β-D-glucopyrannoside（1→6）-β-D-glucopyrannoside]oleanlic acid（12）。结论：化合物6~9,12为首次从该植物中分离得到,化合物5为首次从楤木属植物中分离得到。Objective： To study the chemical constituents from 70% ethanol fraction of Aralia taibaiensis cortex and evaluate its potential in sustainable utilization as medicinal resources from pharmacodynamics. Method：The 3 kg A. taibaiensis cortex medicinal materials were crushed into coarse powder,extracted by 70% ethanol,and concentrated under reduced pressure to obtain extract. The extract was added with appropriate amount of water to get the sample solution which was then adsorbed by 101 macroporous resin column chromatography. Water and70% ethanol were used for elution and two corresponding fractions were obtained. The 70% ethanol fraction was separated and purified by silica gel column chromatography,Sephadex LH-20 gel column chromatography,semipreparative HPLC and recrystallization,and their structures were identified by NMR and MS,physicochemical properties and reference literature. Result： Twelve triterpenoid saponin compounds were obtained and elucidated as araliasaponin Ⅻ（ 1）, chikusetsusaponin 1（ 2）, chikusetsusaponin Ⅳ a（ 3）, tarasaponin Ⅴ（ 4）,yuzhizioside Ⅳ（ 5）,elatoside F（ 6）,araliasaponin Ⅱ（ 7）,araliasaponin Ⅵ（ 8）,araliasaponin Ⅲ（ 9）,araloside A（ 10）, narcissiflorine（ 11）, and 3-O-[β-D-rhamnose（ 1 → 2）-α-L-arabinose]-28-O-[β-Dglucose（ 1 → 6）-β-D-glucose] oleanlic acid（ 12）. Conclusion： Compounds 6-9 and 12 were isolated from Aralia taibaiensis for the first time. Compound 5 was isolated from A. chinensis cortex for the first time.

关 键 词：太白楤木 三萜皂苷 结构鉴定 蓣知子皂苷Ⅳ 

分 类 号：R284.2[医药卫生—中药学] R22[医药卫生—中医学]