HPLC-MS鉴定塔中栽培肉苁蓉药材的17个成分  

Identification of Seventeen Components in Cistanches Herba Cultured in Tarim Desert by HPLC-MS

作　　者：南泽东 任华忠[1] 赵明波[2] 姜勇 屠鹏飞[2] NAN Ze-dong;REN Hua-zhong;ZHAO Ming-bo;JIANG Yong;TU Peng-fei(Leshan Vocational and Technical College,Leshan 614000,China;State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking Universit~ Health Science Center,Belting 100191,China)

机构地区：[1]乐山职业技术学院,四川乐山614000 [2]北京大学天然药物及仿生药物国家重点实验室,北京100191

出　　处：《中国实验方剂学杂志》2018年第20期62-67,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家“重大新药创制”科技重大专项(2012ZX09201201-001,2012ZX09301002-002-002,2012ZX09304-005)

摘　　要：目的：通过HPLC-MS对新疆塔中荒漠栽培肉苁蓉Cistanche deserticola药材60%甲醇提取物的色谱图进行鉴定。方法：采用Agilent ZORBAX Eclipse Plus C_（18）色谱柱（4.6 mm×250 mm,5μm）,以乙腈-水（含0.1%的甲酸）为流动相进行梯度洗脱,流速1 mL·min^（-1）,检测波长254 nm,柱温25℃,进样量10μL。结果：在相同色谱条件下,通过与已分离得到的对照品的保留时间、相对分子质量以及碎片离子进行比较,对新疆塔中荒漠栽培肉苁蓉药材的16个色谱峰中的17个成分进行了准确的鉴定,分别确定为松柏苷,去甲紫丁香苷,紫丁香苷,松果菊苷,肉苁蓉苷A,类叶升麻苷（管花苷A）,肉苁蓉苷B,异类叶升麻苷,2＇-乙酰基肉苁蓉苷A,肉苁蓉苷C,异肉苁蓉苷C,2＇-乙酰基类叶升麻苷,管花苷B,epimeridinoside A,肉苁蓉苷K,肉苁蓉苷J。结论：该研究方法适用于肉苁蓉60%甲醇提取物色谱峰的鉴定,为全面分析该药材的化学成分提供了一定的科学数据。Objective： To identify the chromatographic components of 60% methanol extract in Cistanches Herba cultured in Tarim desert by HPLC-MS. Method： HPLC-MS analysis was performed on a Agilent ZORBAX Eclipse Plus C_（18） column（ 4. 6 mm × 250 mm,5 μm）. The mobile phase was acetonitrile-0. 1% formic acid aqueous solution by gradient elution at the flow rate of 1 mL·min^（-1）,and the detection wavelength was254 nm. The column temperature was set at 25 ℃, and the injection volume was 10 μL. Result： Sixteen chromatographic peaks/seventeen components were accurately identified by comparing the retention time,molecular weights and fragment ions of the compounds we isolated and identified. These chromatographic components were determined as coniferin, demethyl syringin, syringin, echinacoside, cistanoside A,verbascoside（ tubuloside A）, cistanoside B, isoverbascoside, 2＇-acetylcistanoside A, cistanoside C,isocistanoside C,2＇-acetylverbascoside, tubuloside B, epimeridinoside A, cistanoside K and cistanoside J.Conclusion： The HPLC-MS method is suitable for the identification of the chromatographic components of 60%methanol extract in Cistanches Herba. This study will provide the scientific evidence for comprehensively analyzing the chemical constituents of this medicinal herb.

关 键 词：荒漠肉苁蓉 肉苁蓉属 液质联用 甲醇提取物 

分 类 号：R284.1[医药卫生—中药学] R289[医药卫生—中医学]