联合应用抗生素对新生小鼠体重和死亡率的影响  

Effect of antibiotics use on the weight and mortality in newborn mice

作　　者：张欣[1] 李少闻[2] 高小翠 陈嘉彦 ZHANG Xin;LI Shao-wen;GAO Xiao-cui;CHEN Jia-yan(Obstetrics and Gynecology Department;Editorial Department of Chinese Journal of Child Health Care,the Second Affiloated Hospital of Medical School,Xi＇an,Shaanxi 710004,China)

机构地区：[1]西安交通大学第二附属医院妇产科,陕西西安710004 [2]西安交通大学第二附属医院中国儿童保健杂志编辑部,陕西西安710004

出　　处：《中国儿童保健杂志》2018年第10期1088-1091,共4页Chinese Journal of Child Health Care

基　　金：陕西省自然科学基础研究(20145M4144)

摘　　要：目的探讨生命早期使用抗生素对新生小鼠体重和死亡率的影响,为临床治疗提供科学依据。方法选取40只6~8周龄雌性野生型C57BL/6小鼠和20只6~8周龄雄性野生型C57BL/6小鼠,雌性小鼠随机分成对照组和抗生素处理组,每组20只。对照组和抗生素组每2只雌性小鼠配一只雄性小鼠置于一笼中饲养12周。对照组小鼠给予正常饲料和饮水,抗生素组小鼠给予正常饲料和抗生素鸡尾酒组合饮水。每周测量新生鼠体重,检测粪便细菌DNA,统计死亡率。结果抗生素组新生小鼠在生后1周内和1~2周内的总体死亡率均明显高于对照组(P=0.034,0.027);而生后1周内和1~2周内的死亡率无明显性别差异(P>0.05);对照组和抗生素组新生鼠在出生后1周时的体重分别是(3.870±0.079)g、(4.050±0.050)g,两组之间差异无统计学意义(t=1.926,P=0.070)。两周时体重分别是(6.230±0.142)g、(6.770±0.141)g,抗生素新生鼠体重在出生后2周明显高于对照组(t=2.699,P=0.015,);抗生素组新生鼠在1周、2周时的粪便DNA浓度明显低于对照组(P=0.007)。结论新生鼠早期使用抗生素明显减少了肠道细菌数量,导致体重和死亡率增加。Objective To explore the effect of antibiotics use in early life on weight and mortality of newborn mice.Methods A total of 40 female wild-type C57 BL/6 mice aged 6~8 weeks and 20 male wild-type C57 BL/6 mice aged 6~8 weeks were randomly divided into control group and antibiotic treatment group,with 20 mice in each group.Two female mice in the control and antibiotic groups were paired with one male mouse in a cage for 12 weeks.The mice in the control group were given normal feed and water,while the mice in the antibiotic group were given both normal feed and antibiotic cocktail.The weight of newborn mice was measured each week,fecal bacterial DNA was tested,and mortality was counted. Results The overall mortality rate of newborn mice in the antibiotic group was significantly higher than that in the control group（P=0.034,0.027）within 1 week and 1~2 weeks after birth.There was no significantly gender difference in mortality between 1 week and 1~2 weeks after birth（P〉0.05）.The weight of newborn mice in the control group and the antibiotic group at 1 week after birth was（3.870±0.079）g and（4.050±0.050）g,respectively,with no significant difference between the two groups（t=1.926,P=0.070）.At 2 weeks,the weight was（6.230±0.142）g and（6.770±0.141）g,respectively.The weight of the antibiotic newborn mice was significantly higher than that of the control group at 2 weeks after birth（t=2.699,P=0.015）.Moreover,the fecal DNA concentration of newborn mice in the antibiotic group was significantly lower than that of the control group（P=0.007）at 1 week and 2 weeks. Conclusion The use of antibiotics in early life in newborn mice significantly reduces the number of intestinal bacteria,increases weight and mortality,and the mechanism remains to be further studied.

关 键 词：抗生素 联合应用 体重 死亡率 新生鼠 

分 类 号：R179[医药卫生—妇幼卫生保健]