口蹄疫病毒样颗粒活化的肥大细胞对淋巴细胞的调节作用  

Lymphocyte proliferation triggered differentially by the supernatants of mast cells activated with foot-and-mouth disease virus-like particles

作　　者：梁慧婷 赵骏楠 田晓云 李平[1] 杨路达 常枨 李丽敏[1] 王家鑫[1] LIANG Hui-ting;ZHAO Jun-nan;TIAN Xiao-yun;LI Ping;YANG Lu-da;CHANG Cheng;LI L-min;WANG Jia-xin(College of Veterinary Medicine/College of Life Sciences,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学动物医学院/生命科学学院

出　　处：《中国兽医科学》2018年第10期1214-1221,共8页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31402174);河北省高等学校专业综合改革项目;河北省教育厅重点项目(ZD2015040)

摘　　要：为探讨负载口蹄疫病毒样颗粒（VLPs）的肥大细胞对脾初始淋巴细胞增殖的影响,收集小鼠腹腔肥大细胞（pMCs）,以VLPs负载的pMCs为对照组,以丹参酮ⅡA处理后再负载VLPs的pMCs为试验组,不做任何处理的pMCs为空白组。6 h后收集各组pMCs上清液,分别作用于脾初始淋巴细胞,用CCK-8试剂盒检测淋巴细胞增殖,并对pMCs进行甲苯胺蓝染色,观察脱颗粒现象。结果显示,VLPs刺激pMCs可引起明显的脱颗粒,pMCs上清液对脾总淋巴细胞的增殖具有一定的促进作用。丹参酮ⅡA可抑制VLPs诱导的pMCs脱颗粒,并降低pMCs上清液促进淋巴细胞增殖的作用,但VLPs刺激pMCs后的上清液对脾T淋巴细胞增殖具有明显的抑制作用,丹参酮ⅡA处理组pMCs的上清液使脾T淋巴细胞增殖进一步降低。结果表明,VLPs诱导的pMCs脱颗粒产物具有促进脾初始淋巴细胞增殖的作用,但其分泌成分则对脾T淋巴细胞增殖具有抑制作用。To investigate the potential biology of foot-and-mouth disease virus（FMDV）-like particles（VLPs）-activated mast cells（MCs） on the proliferation of lymphocytes in murine spleens, the peritoneal mast cells（pMCs） were harvested. The pMCs pool was randomly divided into three groups.The pMCs pulsed with VLPs were used as a control group,the tanshinoneⅡA-pretreated pMCs pulsed with the VLPs as a test group, and pMCs alone as a blank group.The supernatants were collected from the pMCs culture wells after 6 h for lymphocyte proliferation assay.The pMCs adherent to coverslips were identified by the toluidine blue staining. The pMCs degranulation was substantially induced by VLPs.Consequently,the lymphocyte proliferation of splenic lymphocyte pool was steadily triggered by the supernatants of VLPs-activated pMCs.Of noting,the degranulation of pMCs was significantly inhibited by tanshinoneⅡA,resulting in a remarkable decrease of splenic lymphocyte proliferation. Importantly,the splenic T lymphocyte proliferation was significantly inhibited by the supernatants collected from naive pMCsand VLPs-activated pMCs.Furthermore,T lymphocyte proliferation was very significantly inhibited by the supernatant from tanshinoneⅡA-pretreated pMCs,indicating that granular components released from pMCs pulsed with VLPs are capable of stimulating the proliferation of splenic naive lymphocytes in vitro. However,the secretory components of pMCs loaded with VLPs significantly inhibit splenic T lymphocyte proliferation.Our data reveal a unappreciated role of pMCs in modulation of adaptive immune responses to pathogenic immunogens.

关 键 词：口蹄疫病毒样颗粒 肥大细胞 丹参酮ⅡA 淋巴细胞增殖 

分 类 号：S852.659.6[农业科学—基础兽医学]