广东省人类免疫缺陷病毒1型CRF01＿AE毒株膜区V3环氨基酸序列多态性分析  

Analysis of the polymorphism of envelope protein V3 loop amino acid sequences of HIV-1 CRF01＿AE strains circulating in Guangdong

作　　者：何瑞英 廖宝林[2] 袁小珍[1] 陈伟烈[1] HE Ruiying;LIAO Baolin;YUAN Xiaozhen;CHEN Weilie(Institute of Infectious diseases,Guangzhou Eighth People ＂s Hospital,Guangzhou,Guangdong 510060,China;Infectious Diseases Center,Guangzhou Eighth People ~s Hospital,Guangzhou,Guangdong 510060,China)

机构地区：[1]广州市第八人民医院传染病研究所,广东广州510060 [2]广州市第八人民医院感染病中心,广东广州510060

出　　处：《中国热带医学》2018年第10期984-990,共7页China Tropical Medicine

基　　金：广州市科信局科技惠民专项(No.2014Y2-00149);广东省自然科学基金项目(No.2016A030310108)

摘　　要：目的分析广东省人类免疫缺陷病毒1型(HIV-1)CRF01_AE毒株膜蛋白第三高变区(V3环)氨基酸序列的特点。方法提取HIV-1 CRF01_AE亚型感染者PBMC中前病毒DNA,巢式PCR扩增HIV-1膜基因c2v3c3区域后测序,对V3环氨基酸序列特征进行分析。结果 73份样本与HIV-1 CRF01_AE亚型代表毒株聚集在一起、与其他亚型标准毒株分开,进一步证实本研究73例HIV/AIDS患者感染的HIV-1毒株为CRF01_AE亚型。除6个位点氨基酸一致外,73例病人所有V3环序列的其他29个位点分别有2～8个不同的氨基酸出现;GPGQ为V3环顶端四肽的主要形式,占47.9%,其次为GPGK,占15.1%,四肽中未出现多态性的只有第三位氨基酸;预测HIV-1辅助受体为CCR5的有35例,占47.9%,其次为CXCR4的有34例,占46.6%,两者之间差异无统计学意义(P>0.05)。性传播是广东省CRF01_AE亚型HIV-1感染者的主要感染途径,占54.8%,其次是静脉吸毒,占30.2%。通过性传播途径感染的HIV-1毒株的辅助受体主要为CXCR4,占52.5%,辅助受体为CCR5的占40.0%,两者相比差异无统计学意义(P>0.05)。结论广东省人类免疫缺陷病毒1型CRF01_AE毒株膜区V3环氨基酸存在较高的多态性,使用CCR5及CXCR4为辅助受体的HIV-1毒株比例相近。Objective To investigate the characteristics of V3 loop amino acid sequences of human immunodeficiencyvirus type 1（HIV-1） CRF01_AE strains circulating in Guangdong. Methods DNA was extracted from the peripheral bloodmononuclear cells（PBMCs） of 73 CRF01_AE HIV-1 infected individuals in Guangdong. Nested polymerase chain reaction wasperformed to amplify C2-V3-C3 region of the HIV-1 env genes. The PCR products were directly sequenced and thecharacteristics of V3 loop amino acid sequences were then analyzed. Results The 73 samples were clustered with HIV-1 CRF01_AE subtype representative strains and separated from other subtype standard strains, further confirming that the HIV-1 strain infected in 73 HIV/AIDS patients in this study was CRF01_AE subtype. Sequences analysis showed that only 6 positions of amino acid residue in V3 loop were consistent and the other 29 positions had 2 to 8 different amino acid residuesrespectively. GPGQ was the main form of the V3 ring apical tetrapeptide, accounting for 47.9%, followed by GPGK, accountingfor 15.1%, and only the third amino acid residue was not polymorphic in the tetrapeptide. There was no significant differencebetween the predicted HIV-1 co-receptor of CCR 5（n=35, 47.9%） and CXCR 4（n=34, 46.6%）（P〈0.05）. Sexual transmissionwas the main infection route in the patients infected with CRF01_AE subtype HIV-1 in Guangdong Province, accounting for54.8%, followed by intravenous drug use, accounting for 30.2%. The co-receptors of the HIV-1 strain infected by the sexuallytransmitted route were mainly CXCR4, accounting for 52.5%, followed by 40.0% of CCR5. There was no significant differencebetween them. Conclusions The polymorphism of V3 loop amino acid sequences of HIV-1 CRF01_AE strains circulating inGuangdong is high, and the proportion of HIV-1 strains using CCR5 and CXCR4 as the co-receptors is similar.

关 键 词：人类免疫缺陷病毒1型 CRF01_AE亚型 V3环 序列分析 

分 类 号：R512.91[医药卫生—内科学]