基于线粒体Cytb基因序列的洞庭湖河蚬遗传多样性分析  

Genetic Diversity Analysis of Corbicula fluminea in Dongting Lake Based on the Mitochondrial Cyt b Gene

作　　者：王剑平 朱鹏飞 王健[1] 桂雨婷 李德亮[1] WANG Jian-ping;ZHU Peng-fei;WANG Jian;GUI Yu-ting;LI De-liang(Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province,Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization,College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,P.R.China)

机构地区：[1]湖南农业大学动物科学技术学院水产高效健康生产湖南省协同创新中心湖南省特色水产资源利用工程技术研究中心

出　　处：《水生态学杂志》2018年第5期89-94,共6页Journal of Hydroecology

基　　金：国家自然科学基金项目(31772832);国家公益性行业(农业)科研专项(201503108)

摘　　要：研究洞庭湖不同地理群体河蚬的遗传多样性和遗传结构,为其资源合理开发和有效保护提供参考。在6个样点共采集河蚬126只,以线粒体细胞色素b基因部分序列为分子标记,分析扩增与测序后基因序列的单倍型及多样性、核苷酸多样性,计算群体间遗传分化指数等。获得长531bp的序列片段,共检出5种单倍型,变异位点10个;平均单倍型多样性和核苷酸多样性分别为0.559和0.006。6个河蚬群体间的遗传距离为0.006-0.007;遗传分化指数和AMOVA分析显示,河蚬群体间遗传分化低,遗传变异主要来自群体个体内。最大似然法构建的系统进化树中,单倍型DTH1和DTH5聚为一支,DTH2、DTH3、DTH4聚为另一支。中性检验结果显示洞庭湖河蚬群体相对稳定,没有经历过瓶颈效应和突然的群体萎缩等历史事件。洞庭湖6个河蚬群体间不存在显著的遗传差异,可将其作为一个整体进行管理和保护。In order to better utilize and preserve the Corbicula flumineareserves in Dongting Lake,the genetic diversity and structure of C.flumineafrom six geographical locations were examined using the partial mitochondrial cytochrome b（Cyt b）sequence（531 bp）as the genetic marker.Ten mutations and 521 conservative loci were detected in the Cyt b gene fragments from 126 C.flumineaand five haplotypes were identified（DTH1-5）.The mean haplotype diversity and nucleotide diversity were 0.559 and 0.006,respectively.The genetic distance among the six C.fluminea populations ranged from 0.006 to 0.007.Analysis of the fixation index and molecular variance（AMOVA）showed that the genetic variance was primarily from variation among individuals within populations and there was no significant genetic difference among populations.In the ML phylogenetic tree,the five haplotypes were classified into two clades;the combination of DTH1 and DTH5 form one clade and DTH2-4 the other.The neutral test indicates that the C.fluminea population in the Dongting Lake is relatively stable and has not experienced events such as the bottleneck effect or sudden group atrophy.The results,taken together,show that genetic differences among the six populations are insignificant and C.flumineain the Dongting Lake should be managed and protected as a whole.

关 键 词：河蚬 线粒体细胞色素B 遗传多样性 遗传结构 洞庭湖 

分 类 号：Q347[生物学—遗传学]