肥大细胞功能对脐疗治疗寒凝血瘀型痛经大鼠子宫瞬时受体电位香草酸通道的影响  

Effects of mast cell function on umbilical therapy for uterine transient receptor potential vanillin pathway in rats with dysmenorrhea of cold congelation and blood stasis syndrome

作　　者：李姝婧[1] 孙琰[1] 潘亚辉[1] 赵启韬[1] 高树中[1] 马玉侠[1] LI Shu-jing;SUN Yan;PAN Ya-hui;ZHAO Qi-tao;GAO Shu-zhongz;MA Yu-xia(PHD Student of Class 2016,Shandong University of Chinese Medicine,Jinan 250355,China;Shandong University of Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学2016级博士研究生,济南250355 [2]山东中医药大学,济南250355

出　　处：《中华中医药杂志》2018年第10期4646-4649,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81373721)

摘　　要：目的:观察隔药灸脐、药物贴脐对寒凝血瘀型痛经大鼠扭体反应及子宫组织瞬时受体电位香草酸通道(TRPV)1、TRPV2、TRPV4表达的影响并探讨肥大细胞发挥的功能。方法:将70只成年雌性Wistar大鼠随机分为7组,对照组、模型组、隔药灸脐组、药物贴脐组、色甘酸钠+模型组、色甘酸钠+隔药灸脐组、色甘酸钠+药物贴脐组。除对照组外均造寒凝血瘀型痛经模型,造模后给予相应治疗;采用蛋白免疫印迹法(Western Blot)、实时荧光定量PCR法(Real-time PCR)检测子宫TRPV1、TRPV2、TRPV4蛋白及mRNA表达。结果:脐疗对寒凝血瘀痛经大鼠具有明显镇痛疗效;色甘酸钠有一定镇痛作用;模型组大鼠子宫TRPV1、TRPV2、TRPV4表达较对照组升高(P﹤0.01);脐疗可下调痛经大鼠子宫TRPV1、TRPV2、TRPV4高表达(P﹤0.01,P﹤0.05),隔药灸脐对TRPV调节作用优于药物贴脐;色甘酸钠阻断神阙穴肥大细胞使脐疗镇痛效应及对TRPV表达的调节作用不同程度地削弱。结论:脐疗通过下调痛经大鼠子宫TRPV高表达而达到治疗作用;隔药灸脐对TRPV的调节作用优于药物贴脐;肥大细胞在此效应过程中发挥了重要作用。Objective： To observe the effects of herb-partition moxibustion navel （HM） and drug-coving navel therapy （DC） on writhing response and the expression of TRPV1, TRPV2 and TRPV4 in the uterine and discuss the function of mast cells. Methods： A total of 70 female adult Wistar rats were randomly divided into 7 groups： control group, model group, HM group, CD group, SCG group, SCG＋HM group, SCG＋DC group. In addition to the control group, the other groups were treated with the corresponding treatment after establishing the model of dysmenorrhea with syndrome of cold congelation and blood stasis. Subsequently, the expression of TRPV1, TRPV2 and TRPV4 in the uterus was detected by Western Blot and RT-PCR. Results： Navel therapy has obvious analgesic effect on dysmenorrhea with syndrome of cold coagulation and blood stasis rats; and SCG has certain analgesic effect. The expression of TRPV1, TRPV2 and TRPV4 in the uterus of model group was higher than that of control group （P〈0.01）; Navel therapy reduced the high expression of TRPV 1, TRPV2 and TRPV4 （P〈0.01, P〈0.05） in the uterus of cold coagulation and blood stasis dysmenorrhea rats. The effects of HM on TRPV is better than that of the DC, The blocking of mast cells in the Shen que acupoint by SCG can weaken the analgesic effect of umbilical therapy and the regulation effect on the expression of TRPV in different degrees. Conclusion： Navel therapy achieve therapeutic effect by downregulating the high expression of TRPV in the uterus of dysmenorrhea rats, the effect of HM therapy is superior to DC therapy, and mast cells play an important role in this effect process.

关 键 词：脐疗 肥大细胞 痛经 子宫 瞬时受体电位香草酸通道 

分 类 号：R245[医药卫生—针灸推拿学]