基于以方测证的肾阳虚哮喘大鼠模型建立与评价研究  

Establishment and estimation of the asthma with kidney-yang deficiency rat model based on the syndrome test by prescription

作　　者：郭春荣 施旻昱 庄怡娴 赵杏 李福凤 邹璐 孙祝美 郑幼镭 王慧雯 陈丹 GUO Chun-rong;SHI Min-yu;ZI-IUANG Yi-xian;ZHAO Xing;LI Fu-feng;ZOU Lu;SUN Zhu-mei;ZHENG You-lei;WANG Hui-wen;CHEN Dan(Experiment Center of Teaching ＆ Learning,Shanghai University of Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学教学实验中心,上海201203

出　　处：《中华中医药杂志》2018年第10期4715-4718,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市卫生和计划生育委员会青年科研项目(No.2014JQ028A),上海市协同创新建设项目(No.A1-U16302020102),上海中医药大学第九批大学生科创项目

摘　　要：目的:建立并评价哮喘大鼠肾阳虚病证结合模型。方法:将SD大鼠随机分为正常组、哮喘模型组、肾阳虚哮喘模型组、肾阳虚哮喘+金匮肾气汤组(简称金匮肾气汤组)、肾阳虚哮喘+六味地黄汤组(简称六味地黄汤组)5组,每组10只。除正常组外,其余各组均利用卵蛋白诱发哮喘。肾阳虚哮喘模型组、金匮肾气汤组、六味地黄汤组灌胃地塞米松混悬液2周制作肾阳虚哮喘模型,2周后金匮肾气汤组、六味地黄汤组分别灌胃金匮肾气汤和六味地黄汤治疗。末次给药12h后处死大鼠取材固定,病理切片观察大鼠肺组织病理学变化,荧光免疫组化法测定大鼠肺支气管壁RhoA、Rock2蛋白表达。结果:病理切片显示正常组支气管管壁完整,无炎性渗出。哮喘模型组、肾阳虚哮喘模型组大鼠支气管壁塌陷,周围有大量炎性细胞渗出,且肾阳虚哮喘模型组的病理变化重于哮喘模型组;金匮肾气汤组支气管壁结构塌陷程度较肾阳虚哮喘组明显减轻,周围有少量炎性细胞渗出;六味地黄汤组的病变程度与肾阳虚哮喘模型组无明显差别。荧光免疫组化法显示RhoA、Rock2在各组大鼠支气管壁和肺泡壁均有表达;哮喘模型组RhoA、Rock2蛋白表达显著高于正常组(P﹤0.05),肾阳虚哮喘模型组明显高于哮喘模型组(P﹤0.05),金匮肾气汤组与肾阳虚哮喘模型组比较,RhoA、Rock2表达明显下降(P﹤0.05)。结论:基于以方测证的实验室指标的结果分析判定地塞米松灌胃结合卵蛋白诱发哮喘制造肾阳虚哮喘模型成功。Objective： To estalish and evaluate the asthma with kidney-yang deficiency rat model of combined disease and syndrome. Methods： The SD rats were randomly divided into normal group, asthma model group, asthma of kidney-yang deficiency model group, asthma of kidney-yang deficiency with Jingui Shenqi Decoction group （the following called Jingui Shenqi Decoction group） and asthma of kidney-yang deficiency with Liuwei Dihuang Decoction group （the following called Liuwei Dihuang group）, and each group included 10 rats. Except the normal group, the remaining groups were induced asthma by ovalbumin. The dexamethasone suspension was used for 2 weeks to make the asthma with kidney-yang deficiency model in the asthma of kiduey-yang deficiency model group, Jingui Shenqi Decoction group and Liuwei Dihuang Decoction group. After 2 weeks, the Jingui Shenqi Decoction group and Liuwei Dihuang Decoction group were respectively given Jingui Shenqi Decoction and Liuwei Dihuang Decoction. The rats were executed after 12h of the last treatment, the pathological changes of lung tissue in rats were observed by pathological section, and the protein expressions of RhoA and Rock2 in the pulmonary bronchial wall of rats were measured by immunofluorescence method. Results： The pathological sections showed that the normal group＇s bronchial wall was intact with no inflammatory exudation. There was a large number of inflammatory cell exudation and collapsed bronchial wall in the asthmatic model group and asthma of kidney-yang deficiency model group rats, and the pathological changes were more serious in asthma of kidney-yang deficiency model group. The level of bronchial wall collapse of Jingui Shenqi Decoction group was significantly lower than that in the asthma of kidney-yang deficiency group, with a little inflammatory cell exudation surrounded. There was no significant difference of pathological changes between Liuwei Dihuang Decoction group and asthma of kidney-yang deficiency model group. The immunofluorescence showed tha

关 键 词：慢性哮喘 肾阳虚 以方测证 RHOA ROCK2 模型 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]