MicroRNA-613在人胶质瘤中的表达及其对增殖与转移的影响  

Expression of microRNA-613 in human glioma and its effects on growth and metastasis

作　　者：李传芬 荆文[3] 向冬生 李涛 焦淑欣 杨翠玲 马志红 王敏[1] LI Chuan-fen;JING Wen;XIANG Dong-sheng;LI Tao;JIAO Shu-xin;YANG Cui-ling;MA Zhi-hong;WANG Min(College of Life Sciences,Shandong Normal University,Ji＇nan 250014,China;College of Physical Education,Shandong Normal University,Ji＇nan 250014,China;Electrical Department,Shandong Vocational College,Ji＇nan 250104,China;Department of Microbiology Laboratory,Linyi Disease Prevention and Control Center,Shandong Linyi 276000,China;Department of Neurology,General Hospital of Ji＇nan Military Region,Ji＇nan 250031,China)

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]济南军区总医院神经内科,济南250031 [3]山东师范大学体育学院,济南250014 [4]山东职业学院电气系,济南250104 [5]临沂市疾病预防控制中心微生物检验科,山东临沂276000

出　　处：《解剖学报》2018年第5期579-583,共5页Acta Anatomica Sinica

基　　金：山东省科技重点研发项目(2016GSF201058);山东省自然科学基金青年基金(ZR2014CQ026)

摘　　要：目的探讨microRNA-613(miR-613)在人胶质瘤中的表达及其对增殖与转移的影响。方法采用Real-time PCR法对30例人胶质瘤患者瘤组织和30例非胶质瘤患者的脑组织的miR-613表达水平进行检测,并分析其与胶质瘤临床病理特征及预后的关系;采用Lipofectamine TM2000将miR-613模拟物(miR-613 mimics)和无关序列对照(miR-613 con)转入U87细胞中,Real-time PCR检测两组细胞miR-613的表达水平,CCK8法检测细胞增殖变化,Transwell实验检测细胞侵袭和迁移能力的变化。结果 miR-613在胶质瘤组织中表达低于正常的脑组织(P <0. 05)。胶质瘤组织miR-613的表达水平与病理分级成负相关,与生存时间成正相关(P <0. 05);与miR-613con比较,miR-613 mimics细胞增殖水平、侵袭与迁移能力显著降低(P <0. 05)。结论 miR-613具有抑制胶质瘤细胞的生长、侵袭与迁移的作用,有望成为胶质瘤的潜在治疗靶点。Objective To investigate the expression of microRNA-613（ miR-613） in human glioma and its effect on the proliferation and metastasis. Methods Thirty human glioma patients tissues and 30 Non-glioma patients tissues were detected by Real-time PCR,and to analysie their relationship with clinicopathological characteristics of gliomas. MiR-613 mimics and unrelated sequence control（ miR-613 con） were transfected into U87 cells using Lipofectamine TM2000. The expression of miR-613 in the two groups of cells was detected by Real-time PCR,the proliferation of cells was detected by CCK8 assay,and the invasion and migration of cells were detected by Transwell assay. Results The expression of miR-613 in Glioma tissues was lower than that in corresponding paracancerous tissues（ P〈0. 05）. The expression level of miR-613 in glioma tissue was negatively correlated with pathological grade and positively correlated with survival time（ P〈0. 05）. Compared with miR-613 con cells,miR-613 mimics cells proliferation,invasion and migration were significantly decreased（ P〈0. 05）. Conclusion MiR-613 can inhibit the proliferation,invasion and migration of glioma cells,and it might be a potential therapeutic target for the treatment of glioma.

关 键 词：microRNA-613 胶质瘤 U87 生长 转移 实时定量聚合酶链反应 人 

分 类 号：R739.41[医药卫生—肿瘤]