DCs及其膜糖蛋白在慢性淋巴细胞性甲状腺炎中的临床研究  

Clinical Study of DCs and Its Membrane Glycoprotein in Hashimoto's Thyroiditis

作　　者：杜静 赵迪[2] 万晓娟 王淑雅[1] 刘理峰 Du Jing;Zhao Di;Wan Xiaojuan(Department of Endocrinology,The Yellow River Central Hospital,Henan 450003,China)

机构地区：[1]黄河中心医院内分泌科,郑州450003 [2]郑州大学第一附属医院内分泌科,450052 [3]广东省人民医院内分泌科,510080

出　　处：《医学研究杂志》2018年第9期165-167,175,共4页Journal of Medical Research

摘　　要：目的就甲状腺组织树突状细胞(DCs)数量及患者外周血DCs细胞膜糖蛋白变化在慢性淋巴细胞性甲状腺炎(HT)患者病理过程中的意义进行分析研究。方法研究选取确诊HT患者113例,为观察组,同时选取同期健康体检者120例作为对照组;收集患者手术中的甲状腺组织以及外周血进行DCs计数以及DCs膜糖蛋白Galectin-9表达情况进行分析讨论。结果本研究中观察组受试对象113例,对照组120例受试对象基本情况可比性良好。观察组患者分型参照Woolner's分类法,包含L-型(lymphoid type) 39例,O-型(oxyphilic type) 37例,P-型(pronounced type) 37例。L-型HT患者DCs细胞计数为(6. 54±1. 37)×10~6/L,O-型HT患者DCs细胞计数为(9. 55±1. 08)×10~6/L,P-型HT患者DCs细胞计数为(3. 59±1. 33)×10~6/L,全部HT患者的平均DCs细胞计数为(7. 27±1. 35)×10~6/L;均高于对照组的水平(1. 12±0. 34)×10~6/L,差异均有统计学意义(P <0. 05)。使用流式细胞仪检测DCs中Galectin-9蛋白表达比例水平,结果提示L-型HT患者DCs中Galectin-9蛋白表达比例为24. 66%,O-型HT患者DCs中Galectin-9蛋白表达比例为21. 29%,P-型患者为20. 54%,全部患者的平均DCs中Galectin-9蛋白表达比例则为24. 32%;均高于对照组的7. 20%。同时,DCs膜表面糖蛋白Galectin-9表达的Western blot法检测结果也提示观察组显著高于对照组。结论在HT病理过程中存在者DCs细胞的参与,故可见几种常见类型患者DCs细胞计数均高于健康对照组;同时健康人DCs中Galectin-9蛋白量表达高于HT患者水平,可能与HT患者存在过度免疫有关。Objective To analyze the significance of the number of DCs in thyroid tissue and the changes of DCs in peripheral blood of patients with Hashimoto ′s thyroiditis. Methods A total of 113 patients with HT were selected as the observation group and 120 healthy subjects were selected as the control group. The thyroid tissue and the peripheral blood were collected and the expression of Galectin-9 was detected. Results In the study group, 113 cases were observed in the observation group and 120 cases in the control group were comparatively good. In the observation group, the patients were classified into 35 cases （lymphoid type）, 37 cases of O-type （oxyphilic type） and 37 cases of P-type（pronoumced） type （6.54±1.37）×10 6/L. DCs cells in O-type HT patients were （9.55±1.08）×10 6/L, DCs in P-type HT patients were （3.59±1.33）×10 6/L, and the mean of all patients with HT DCs were （7.27±1.35）×10 6/L, which were higher than those in the control group （1.12± 0.34）×10 6/L. The difference was statistically significant （ P 〈0.05）. The expression of Galectin-9 protein in DCs was 24.66% in DCs of L-type HT patients, and the expression of Galectin-9 protein in DCs of O-type HT patients was 21.29% % And P-type patients were 20.54%. The proportion of Galectin-9 protein in the mean DCs of all patients was 24.32%, which was higher than that of the control group （7.20%）. At the same time, the expression of Galectin-9 in DCs was also significantly higher than that in the control group. Conclusion The number of DCs cells in several common types of patients is higher than that of healthy controls. The expression of galectin-9 protein in healthy human DCs is higher than that in HT patients, which may be related to HT patients Excessive immunity is associated.

关 键 词：树突状细胞 细胞膜糖蛋白 慢性淋巴细胞性甲状腺炎 

分 类 号：R581[医药卫生—内分泌]