不同发育时间蓖麻种子DNA甲基化的MSAP分析  

MSAP Analysis of DNA Methylation of Castor Seed at Different Developmental Stages

作　　者：黄凤兰[1,2,3,4] 陈晓凤 杨丽凤[1] 常如慧[1] 李国瑞 狄建军[1,2,3,4] 徐雅楠 孙洪玲[1] 隋久香 孙雪 孟祥越 HUANG Feng-lan;CHEN Xiao-feng;YANG Li-feng;CHANG Ru-hui;LI Guo-rui;DI Jian-jun;XU Ya-nan;SUN Hong-ling;SUI Jiu-xiang;SUN Xue;MENG Xiang-yue(College of Life Sciences,Inner Mongolia University for Nationalities,Tongliao 028043,China;Engineering Research Center for Castor Industry of Inner Mongolia Universities,Tongliao 028000,China;Key Laboratory for Castor Breeding of Inner Mongolia,Tongliao 028000,China;Collaborate Innovation Cultivate Center for Castor Industry of Inner Mongolia,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学生命科学学院,内蒙古通辽028043 [2]内蒙古自治区高校蓖麻产业工程技术研究中心,内蒙古通辽028000 [3]内蒙古自治区蓖麻育种重点实验室,内蒙古通辽028000 [4]内蒙古自治区蓖麻产业协同创新培育中心,内蒙古通辽028000

出　　处：《内蒙古民族大学学报（自然科学版）》2018年第4期319-323,357,共6页Journal of Inner Mongolia Minzu University：Natural Sciences

基　　金：国家自然科学基金项目(31160290;31460353;31860071);国家青年科学基金项目(31401418);内蒙古自治区自然科学基金面上项目(2017MS0339);内蒙古自治区科技创新引导奖励资金项目(KJCX15002);内蒙古自治区"草原英才"计划项目(201511);内蒙古自治区草原英才创新团队支持项目(2017);内蒙古自治区蓖麻育种重点实验室开放基金项目(MDK2016031);内蒙古自治区蓖麻育种重点实验室开放基金项目(MDK2016030);内蒙古自治区高校蓖麻产业工程技术研究中心开放基金项目(MDK2017030;MDK2018014);内蒙古自治区高校蓖麻产业工程技术研究中心开放基金项目(BMYJ2015-07;MDK2016008);内蒙古自治区研究生教育创新计划资助项目(S20171013604);内蒙古民族大学研究生科研项目(NMDSS1755);内蒙古民族大学2017研究生重点建设课程项目(生物工程技术原理)

摘　　要：为了分析不同发育时间蓖麻种子DNA甲基化的变化,本研究采用MSAP技术以发育10,20,30,40,50,60 d蓖麻种子为材料进行甲基化分析.结果表明,在10~60 d发育的种子中总甲基化率为19.77%~41.18%,全甲基化率为7.99%~24.16%,半甲基化率为7.56%~25.88%.发育20 d的种子中甲基化率最高,20~40 d的种子总甲基化率、全甲基化率、半甲基化率均表现出逐渐下降.在种子发育过程中,共鉴定出647条发生甲基化的条带,然而仅有37条序列是与已知序列同源,包括磷脂转运ATP酶、磷酸酶、甲基转移酶等,与脂肪酸的合成、运输有一定的联系.MSAP was used to analyze the correlation of DNA methylation in 10, 20, 30, 40, 50 and 60 day oldcastor seeds. The total methylation rates were 19.77%-41.18%, permethylation rates were 7.99%-24.16%, and hemimethylation rates were 7.56%-25.88% in the castor bean seeds that developed in 10-60 days. The highestmethylation rate was found in seeds with 20 day development. The total methylation rate, permethylation rate and hemimethylation rate of seeds showed a gradual decrease from 20 to 40 days. A total of 647 bands were identified with different methylation or demethylation patterns during seed maturation. However, only 37 sequences were homologous to known proteins, including phospholipid-transporting ATPase, phosphatases, methyltransferase which are related in the synthesis and transportation of fatty acids. These results provide a foundation for the study of castor fatty acid.

关 键 词：蓖麻 MSAP 差异基因分析 

分 类 号：S565.6[农业科学—作物学]