妇女痛经颗粒中丹参酮Ⅱ_A的含量测定  

Determination of Tanshinone Ⅱ_A in Funvtongjing Particles by HPLC

作　　者：丁叶 孙茜 Ding Ye;Sun Qian(Renhe Hospital of baoshan District,Shanghai 200431)

机构地区：[1]上海市宝山区仁和医院,上海200431

出　　处：《江西化工》2018年第5期77-80,共4页Jiangxi Chemical Industry

摘　　要：建立妇女痛经颗粒中丹参酮ⅡA的含量测定方法。采用HPLC法,色谱柱:Diamonsil C18色谱柱(250mm×4. 6mm,5μm),以甲醇-水(75:25)为流动相,流速为1. 0mL·min^(-1),柱温35℃,UV检测波长为270nm,进样量为10μl,测定7批妇女痛经颗粒中丹参酮ⅡA的含量。在此色谱条件下,丹参酮ⅡA可与其他成分较好的分离,峰形对称,丹参酮ⅡA对照品在0. 6128~76. 6μg·mL^(-1)范围内与峰面积呈良好的线性关系(R^2=0. 9999)。样品平均回收率为97. 56%,RSD为1. 25%,精密度、稳定性、重复性的RSD均〈2%。该方法灵敏快速,操作简便,重现性好,可用于测定妇女痛经颗粒丸中丹参酮ⅡA的含量。To establish the assay of Tanshinone Ⅱ A in Funvtongjing Particles by high performance liquid chromatography （ HPLC ）. Seven batches of Funvtongjing Particles were determined for the content of Tanshinone Ⅱ A by HPLC. The samples were separated on Diamonsil C18 （250mm × 4.6mm ,5μm） at：the column temperature was 35 ℃ , with the mobile phase composed of Methanol -water（75：25 ） at a flow of 1.0mL ·min-1. The detection wavelength was 270 nm and the sampling volume was 10 μl. It was found that Tanshinone Ⅱ A eould be isolated from the other components and the type of peak was symmetrical. Tanshinone Ⅱ A showed a good liner relationship with the peak area at the rage of 0.6128 - 76.6μg · mL-1 of Tanshinone Ⅱ A （R2 = 0. 9999）. The average reeovelT of Tanshinone Ⅱ A was 97.56% and RSD was 1.25% , the RSDS of precision, stability, repeatability were less than 2%. This method was shown to be suitable for the determination of the content of Tanshinone Ⅱ A in Funvtongjing Particles.

关 键 词：妇女痛经颗粒 丹参酮ⅡA HPLC 

分 类 号：R286.0[医药卫生—中药学]