黄酮类化合物与β-乳球蛋白相互作用的电喷雾质谱研究  

Study on Interaction between Flavonoids and β-Lactoglobulin by Electrospray Ionization Mass Spectrometry

作　　者：徐康宁 田淑珍 白斌 贾婧[1] 甘泉涌[1] 陈强[1] XU Kang ning;TIAN Shu zhen;BAI Bin;JIA Jing;GAN Quan yong;CHEN Qiang(School of Medical,Xiangyang Vocational and Technical College,Xiangyang 441021,China)

机构地区：[1]襄阳职业技术学院医学院,湖北襄阳441021

出　　处：《化学与生物工程》2018年第10期64-68,共5页Chemistry & Bioengineering

摘　　要：采用高效液相色谱-电喷雾-四极杆-飞行时间高分辨质谱(HPLC-ESI-Q-TOF-MS)首次研究了黄酮类化合物与β-乳球蛋白的相互作用,通过直接计算法测定了β-乳球蛋白与3种黄酮小分子(桑色素、槲皮素、杨梅苷)在不同体积比时相互作用的结合常数。结果表明,不同体积比下,桑色素、槲皮素、杨梅苷均能与β-乳球蛋白形成稳定的复合物;单个β-乳球蛋白结合的黄酮小分子数目随着体积比的减小(黄酮小分子比例的增大)而增加,结合常数数量级为103~105,结合能力较强;3种黄酮小分子与β-乳球蛋白的结合能力大小依次为:桑色素>槲皮素>杨梅苷。We studied the interaction between flavonoids and β -lactoglobulin for the first time by HPLC-ESI-Q-TOF-MS,and measured the binding constants of β -lactoglobulin with three flavonoids（morin,quercetin,and myricetrin） at different volume ratios by a direct calculation method.The results indicate that morin,quercetin,and myricetrin can form stable complexes with β -lactoglobulin at different volume ratios.The binding numbers increase with the decreasing of volume ratios（ i.e. increasing of flavonoids）,and the binding constants are measured to be around 10 3-10 5,so the binding abilities are reasonably strong.The sequence of binding abilities of three flavonoids with β -lactoglobulin is：morin〉quercetin〉myricetrin.

关 键 词：Β-乳球蛋白 电喷雾质谱 黄酮类化合物 

分 类 号：O675.63[理学—化学]