蔗糖合成酶(SUS)基因的SNP标记开发及其与淀粉性状的关联分析  

SNP Marker Development and Association Analysis with Starch Characteristics for Sucrose Synthase(sus) Gene in Maize

作　　者：葛兆鹏 张莎莎 赵美爱[2,3] 裴玉贺 郭新梅[1,2] 宋希云 GE Zhao-peng;ZHANG Sha-sha;ZHAO Mei-ai;PEI Yu-he;GUO Xin-mei;SONG Xi-yun(College of Agronomy,Qingdao Agricultural University,Qingdao 266109;Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application,Qingdao 266109;College of Life Sciences,Qingclao Agricultural University.Oin~dao 266109.China~)

机构地区：[1]青岛农业大学农学院,青岛266109 [2]青岛市主要农作物种质资源创新与应用重点实验室,青岛266109 [3]青岛农业大学生命科学学院,青岛266109

出　　处：《玉米科学》2018年第5期37-43,48,共8页Journal of Maize Sciences

基　　金：国家自然科学基金项目(No.31201218);山东省现代农业产业技术体系玉米产业创新团队首席专家项目(No.SDAIT-02-01)

摘　　要：通过直接测序法获得玉米亲本（H21和紫糯96-619）susl基因全长序列并进行亲本间序列比对，得到120个SNP位点，通过高分辨率熔解曲线（HRM）对300个近等基因系H21BC，F2：3进行SNP位点分型，并将后代的基因型与群体的直链淀粉含量和淀粉糊化特性进行关联分析。结果表明，marker31与直链淀粉含量和淀粉崩解值、峰值时间、糊化特性均连锁紧密；marker17与直链淀粉含量和淀粉的峰值时间、淀粉最终黏度均连锁紧密；marker12与直链淀粉含量、淀粉的峰值时间连锁紧密。The full-length sequences of sucrose synthase l（susl） gene in H21 and ZN96-619 were obtained by direct-sequencing. Then sequence alignment between them was conducted, and 120 single nucleotide polymorphism （SNP） loci were found. Through high resolution melting-curve（HRM）, the 300 near-isogenic lines（NIL） in H21 BCsF2：3, derived from cross of H21 x ZN 96-619, were genotyped, and the correlation analysis of the genotypes and the amylose content and starch pasting property of the NIL population were conducted. Among the 120 SNP loci, three markers were highly associated with amylose or starch pasting properties, with marker 31 being highly related to amylose content, starch slaking value, peak time and pasting properties, marker 17 being highly related to amylose content, peak time and final viseositv, and marker 12 closelv related to amylose content and peak time.

关 键 词：玉米 序列分析 susl基因 单核苷酸多态性 关联分析 

分 类 号：S513.035.3[农业科学—作物学]