文冠果壳提取物体外抑制HepG_2细胞增殖活性部位筛选研究  

Screen of Activity Part of Inhibiting Proliferation of HepG_2 Cell Lines from Xanthoceras sorbifolia Shell

作　　者：张严磊 施欢贤 李世映 段金廒 宋忠兴 唐志书 ZHANG Yan-lei;SHI Huan-xian;LI Shi-ying;DUAN Jin-ao;SONG Zhong-xing;TANG Zhi-shu(Shaanxi University of Chinese Medicine/Shaanxi Collaborative Innovation Center of Industrialization of Traditional Chinese Medicine Resources,Xianyang 712083,China)

机构地区：[1]陕西中医药大学/陕西省中药资源产业化协同创新中心,陕西咸阳712083

出　　处：《中国现代中药》2018年第9期1083-1086,共4页Modern Chinese Medicine

基　　金：陕西省重点产业创新链(群)--社会发展领域项目(2018ZDCXL-SF-01-06)

摘　　要：目的:筛选文冠果壳体外抑制肝癌Hep G2细胞增殖活性部位。方法:分别用水、10%、30%、50%、70%、95%的乙醇水溶液,从文冠果壳中提取得到A、B、C、D、E和F 6个提取部位(XSHE),采用MTT法来筛选抑制肝癌细胞HepG2增殖的活性部位。结果:显示6个提取部位均有抑制HepG2细胞增殖作用,其中提取部位F(95%乙醇水溶液提取物)抑制HepG2细胞增殖作用最强,当95%XSHE给药质量浓度为75. 00μg·mL^(-1)时,其对HepG2细胞增殖的抑制率高达(80. 44±2. 33)%,高于等浓度的阳性对照丝裂霉素C(MMc),且Hep G2细胞在95%XSHE干预下形态发生变化,具体表现为细胞皱缩,细胞间隙变大,从培养板脱落等作用。结论:文冠果壳提取物在体外具有良好的抑制Hep G2增殖的生物活性,为我们进一步探索文冠果壳提取物抗肿瘤作用机制奠定了基础。Objective： To screen the activity part of inhabiting the multiplication of Hep G2 cell line from Xanthoceras sorbifolia shell. Method： X. sorbifolia shell was extracted using water,10%,30%,50%,70% and 95% ethanol,and six different parts A,B,C,D,E and F were obtained,which were exerted in the experiment of inhabiting the multiplication of Hep G2 cell line by MTT method. Results： The results showed that all extracts of X. sorbifolia shell showed activity of inhibiting the proliferation of cells of Hep G2,among them the section F possessed the strongest antitumor activity,the inhibition of part F reached as high as 80. 44%. Conclusion： X. sorbifolia shell extract has good biological activity in inhibiting the proliferation of Hep G2 in vitro,which has laid a foundation for further explore the anti-tumor mechanism of X. sorbifolia shell extract.

关 键 词：文冠果壳提取物 抑制HepG2细胞增殖 筛选 

分 类 号：R285[医药卫生—中药学]