傣药人面果叶中总黄酮含量测定方法研究  

Determination of Total Flavonoids from Leaves of Garcinia xanthochymus

作　　者：刘慧[1] 钟芳芳 谭雪 卢青秀 杨光忠[3] 陈玉[1] LIU Hui;ZHONG Fang-fang;TAN Xue;LU Qing-xiu;YANG Guang-zhong;CHEN Yu(College of Chemistry and Materials Science,South Central University for Nationalities,Wuhan 430074,China;Wenzhou Dongtou Product Quality Supervision and Testing Institute,Wenzhou 325000,China;National Demonstration Center for Experimental Ethnopharmacology Education,South-Central Universityfor Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学化学与材料科学学院,湖北武汉430074 [2]温州市洞头区产品质量监督检验所,浙江温州325000 [3]中南民族大学民族药学国家级实验教学示范中心,湖北武汉430074

出　　处：《中国现代中药》2018年第9期1105-1109,共5页Modern Chinese Medicine

基　　金：国家自然科学基金资助项目(31370379);国家重大新药创制科技重大专项(2017Z09301060)

摘　　要：目的:建立人面果叶总黄酮含量测定的方法。方法:比较Na NO_2-Al(NO_3)_3-Na OH和Al Cl_3-CH_3COONa/CH_3COOH两种显色方法测定人面果叶中总黄酮含量的差异。结果:用Al Cl_3-CH_3COONa/CH_3COOH显色的方法,供试品和对照品Fukugetin在415 nm处显示有较强和稳定的吸收。结论:采用Al Cl_3-CH_3COONa/CH_3COOH比色法测定人面果叶总黄酮的含量,并建立了该方法测定人面果叶总黄酮含量的最优条件。Objective：To establish a method for determination of total flavonoids from the leaves of Garcinia xanthochymus.Methods：Na NO2-Al（NO3）3-Na OH and Al Cl3-CH3COONa/CH3COOH colorimetry methods were compared to determine the total flavonoids from the leaves of G.xanthochymus.Results：Both the test sample and Fukugetin showed strong and stable absorptions at 415 nm by Al Cl3-CH3COONa/CH3COOH colorimetry.Conclusion：It is reasonable to determine the total flavonoids by using Al Cl3-CH3COONa/CH3COOH colorimetry,the optimum condition of this method was selected.

关 键 词：人面果叶 总黄酮 含量测定 紫外-可见分光光度法 

分 类 号：R29[医药卫生—民族医学]