不同浓度胆巴液浸泡下附子中生物碱含量变化研究  

Content Variation of Alkaloids Components in Aconiti Lateralis Radix Praeparata Treated with Different Concentration of Calcium Chloride Solution

作　　者：杨千千 郝学贤 张辰露[3] 梁宗锁[1] YANG Qian-qian;HAO Xue-xian;ZHANG Chen-lu;LIANG Zong-suo(Zhejiang Sci-Tech University College of Life Science,Hangzhou 310018,China;Shaanxi Huake Biological Pharmaceutical Co.Ltd,Hanzhong 723000,China;Shaanxi Sci-Tech University,Hanzhong 723000,China)

机构地区：[1]浙江理工大学生命科学学院,浙江杭州310018 [2]陕西华克生物医药有限公司,陕西汉中723000 [3]陕西理工大学,陕西汉中723000

出　　处：《中国现代中药》2018年第9期1137-1141,1145,共6页Modern Chinese Medicine

基　　金：国家科技部十二五科技支撑项目(2015BAC01B03);国家中医药管理局标准化建设(ZYBZH-Y-QIN-35)

摘　　要：目的:为了规范附子加工工艺,研究附子在胆巴液浸泡过程中6种酯型生物碱的含量变化。研究方案设定了胆巴液的浓度及浸泡时间,为优化附子的泡胆过程提供依据。方法:采用高效液相色谱法测定附子中6种酯型生物碱的含量。流动相为乙腈-四氢呋喃(A,25∶15)和0. 1 mol·L^(-1)醋酸铵溶液(B),洗脱梯度为0～48 min,15%～26%A; 48～49 min,26%～35%A; 49～58 min,35%A; 58～65 min,35%～15%;检测波长为235 nm。结果:胆巴液浓度≥15%时,采挖后的泥附子中苯甲酰新乌头原碱、新乌头碱、次乌头碱和乌头碱的含量均呈下降趋势,且浓度越高,下降越明显。整体下降趋势在18 d以后趋于平缓,在浸泡第18 d后分别下降60%～80%,20%～50%,75%～85%,20%～60%。结论:不同浓度的胆巴液浸泡下,附子的质量变化不同。其中,在10%浓度的胆巴液中浸泡,质量不稳定,在20%及以上浓度的胆巴液中泡胆,附子的质量相对稳定。在胆巴液浸泡过程中附子药材的效/毒比也在发生变化,在18～24 d期间,呈现出活性成分较高,而毒性成分较少的状态,有利于实际生产。Objective： To study the content variation of 6 alkaloids in Aconiti Lateralis Radix Praeparata treated with calcium chloride solution,and provide the basis for processing of Aconitum carmichaelii. Methods： Six alkaloids were determined by HPLC. The mobile phase consisted of acetonitrile-tetrahydrofuran（ A,25： 15） and 0. 1 mol·L-1 ammonium acetate solution（ B）. The elution gradient was 0-48 min,15%-26% A; 48-49 min,26%-35% A; 49-58 min,35% A; 58-65 min,35%-15%. Results： When the concentration of calcium chloride aqueous solution was ≥15%,the content of benzoyl neatidine,neaconitine,hypaconitine and aconitine decreased,the decrease was more obvious with the increase of calcium chloride aqueous solution concentration. The overall downward trends became flat after 18 days,and the four alkaloids decreased by60%-80%,20%-50%,75%-85% and 20%-60% respectively after soaking for 18 days. Conclusion： The quality of Aconiti Lateralis Radix Praeparata changed in different concentrations of calcium chloride solution. Among them,and the quality was not stable soaking in 10% calcium chloride solution,soaking concentration of 20% or more calcium chloride,the quality of Aconiti Lateralis Radix Praeparata was more stable. The effect/toxicity ratio of Aconiti Lateralis Radix Praeparata in the process of soaking in calcium chloride solution was also changed. During the period from 18 days to 24 days,the active ingredients were higher and the toxic components were less.

关 键 词：附子 胆巴溶液 生物碱 效/毒比 

分 类 号：R284.1[医药卫生—中药学]