白芨多糖对围绝经期大鼠卵巢VEGF、血清MDA、子宫内膜形态学的影响  

Effects of bletilla striata polysaccharideon on expression of vascular endothelial growth factor in rat ovaries and malondialdehyde in rat serum and endometrial morphology during perimenopause period

作　　者：颜文斌 曾祥涛 戴孟诗 叶杭 房源 黄春霞[1] YAN Wen-bin;ZENG Xiang-tao;DAI Meng-shi;YE Hang;FANG Yuan;HUANG Chun-xia(Basic Medicine,Changsha Medical University School,Changsha Hunan 410219,China)

机构地区：[1]长沙医学院基础医学院,湖南长沙410219

出　　处：《局解手术学杂志》2018年第10期698-701,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：湖南省大学生研究性学习和创新性实验计划项目[湘教通(2017)205号文件-840号]

摘　　要：目的观察白芨多糖对围绝经期大鼠卵巢血管内皮生长因子(VEGF)、血清丙二醛(MDA)、子宫内膜形态学的影响。方法采用自然老化法建立围绝经期大鼠模型。12~14月龄SD雌性大鼠40只,随机分为低、中、高剂量白芨多糖(0. 5 g/kg,1 g/kg,2 g/kg)及老年组,灌胃给药8周。免疫蛋白印迹法检测卵巢VEGF蛋白含量变化;用TBA法检测血清丙二醛(MDA)含量;HE染色法观察大鼠子宫内膜形态学。结果用药8周后,与老年对照组比较,VEGF蛋白表达在白芨多糖低、中、高剂量组中表达呈阶梯性升高,血清MDA含量在白芨多糖低、中、高剂量组中表达呈阶梯性降低,差异有显著统计学意义(P <0. 01),并存在剂量依赖性,子宫内膜随剂量的增加而出现内膜层增厚以及腺体增多。结论白芨多糖可通过升高卵巢VEGF蛋白表达、降低血清MDA的水平,改善子宫功能,从而延缓大鼠围绝经期的进程。Objective To investigate the effect of the bletilla striata polysaccharide on the expression of ovarian vascular endothelial growth factor（ VEGF） and serum malondialdehyde（ MDA） and endometrial morphology in rat during perimenopause period. Methods The animal model of perimenopause rats was established by unforced aging. A total of 40 female SD rats（ 12 ~ 14 months old） were randomly divided into four groups： Chinese medicine low（ 0. 5 g/kg）,medium（ 1 g/kg）,high（ 2 g/kg） dosage group and the elder model group. The rats were given with corresponding drugs for 8 weeks. The content changes of ovarian VEGF protein was detected by western blot; serum MDA content was detected by thiobarbituric acid; the morphology of endometrium in rats was observed by HE staining. Results Eight weeks later,compared with the elder model group,the VEGF protein expression in Chinese medicine low,medium,high dosage group increased,the serum MDA content in Chinese medicine low,medium,high,dosage group reduced,the differences were statistically significant（ P〈 0. 01）,with dose dependence. Endometrial thickening and glandular increasing with the increase of dose. Conclusion Bletilla striata polysaccharide can upregulate the expression of VEGF protein,and down regulate the level of serum MDA,which improves the uterine function so as to delay the process of perimenopause in rats.

关 键 词：白芨多糖 VEGF MDA 子宫内膜 围绝经期 卵巢 

分 类 号：R392.5[医药卫生—免疫学] R271[医药卫生—基础医学]