沉香样品中曲霉属真菌菌株HNWSW-20的分离鉴定及其次生代谢产物的研究  

Isolation and Identification of the Fungus Aspergillus sp. HNWSW-20 from Chinese Agarwood and Its Secondary Metabolites

作　　者：邓加艾 戴好富[2] 王宇光[2] 陈惠琴[2] 谭志琼 梅文莉[2] DENG Jiaai;DAI Haofu;WANG Yuguang;CHEN Huiqin;TAN Zhiqiong;MEI Wenli(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou,Hainan 570228,China;Institute of Tropical Biosci-ence and Biotechnology,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Tropical Crops,Ministry of Agriculture,Haikou,Hainan 571101,China;School of Basic Medicine and Life Science,Hainan Medical University,Haikou,Hainan 571199,China)

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101 [3]海南医学院基础医学与生命科学学院,海南海口571199

出　　处：《热带作物学报》2018年第8期1618-1624,共7页Chinese Journal of Tropical Crops

基　　金：海南省耕地改良关键技术研究与示范专项"水稻-瓜菜-绿肥等轮作模式研究与推广"(No.HUNGDg1201504);2016年海南省研究生创新基金项目(No.HyS-2016-20);中国热带农业科学院环境与植物保护研究所中央级公益性科研院所基本科研业务费/创新团队与人才培育专项资助(No.2016hzs1J027);中国热带农业科学院热带生物技术研究所基本科研业务费专项(No.ITBB2015RC02);海南省自然科学基金创新研究团队项目(No.2017CXTD020)

摘　　要：本文采用平板分离法从沉香样品中分离得到一株真菌HNWSW-20,从形态学和分子生物学上鉴定其为曲霉菌(Aspergillus sp.),并利用多种色谱技术对其次生代谢产物进行分离纯化,根据波谱数据和理化性质鉴定其化合物结构为:2?,3?-dihydrosorbicillin(1),sorbicillin(2),2,3-二氢-2-(1-丙烯)-6,8-二甲基-7-羟基-色酮(3),邻苯二甲酸二丁酯(4),7-羟基-异苯并呋喃-1(3H)-酮(5)二十烷酸甲酯(6);分别采用Ellman比色法和pNPG法测定化合物的乙酰胆碱酯酶抑制活性和α-葡萄糖苷酶抑制活性,结果显示上述化合物1、2、4具有乙酰胆碱酯酶抑制活性,而化合物1、3、5具有α-葡萄糖苷酶抑制活性。本文中化合物1~2为首次从曲霉属中分离得到,并首次报道具有乙酰胆碱酯酶和α-葡萄糖苷酶抑制活性。The fungus HNWSW-20 from Chinese agarwood was isolated by a plate separation method and identified as Aspergillus sp. by morphology and molecular biology. The secondary metabolites were isolated and purified by various chromatographic technique, and the structures of the compounds were identified as 2＇,3＇-dihydrosorbicillin（1）, sorbicillin（2）,（2 R）-2,3-dihydro-7-hydroxyl-6,8-dimethyl-2-[（E）-prop-1-enyl] chromen-4-one（3）, dibutylphthalate（4）, 7-hydroxy-1（3 H）-isobenzofuranone（5）, eicosanoic acid（6） on the basis of spectroscopic evidence and physicochemical properties. The acetylcholinesterase and α-glucosidase inhibitory activities of the compounds were determined by Ellman colorimetric assay and pNPG method, respectively. Compounds 1, 2 and 4 showed acetylcholinesterase inhibitory activities, whereas compounds 1, 3 and 5 exhibited α-glucosidase inhibitory activities. In addition, compounds 1–2 were for the first time isolated from Aspergillus sp., and this was the first report about their acetylcholinesterase and α-glucosidase inhibitory activities.

关 键 词：沉香 真菌 分离鉴定 次生代谢产物 生物活性 

分 类 号：R284.1[医药卫生—中药学]