猪呼吸道疾病综合征主要致病菌的分离鉴定  

Isolation and identification of main pathogens of porcine respiratory disease complex

作　　者：王一涵 周宏超[1] 罗丽华 冯秀 赵紫印 邢福珊[1] 郭抗抗[1] WANG Yi-han Kang kang;ZHOU Hong-chao;LUO Li-hua;FENG Xiu;ZHAO Zi-yin;XING Fu-shan;GUO(College of Veterinary Medicine,Northwest A ＆ F University,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学动物医学院

出　　处：《中国兽医学报》2018年第10期1893-1900,共8页Chinese Journal of Veterinary Science

基　　金：陕西省重点农业科技推广及示范资助项目(ZDKJ2014-33)

摘　　要：对3个猪场的4头呼吸道疾病综合征病猪肺脏（猪场分别编号A、B、C,其中猪场A 1头、猪场B 2头、猪场C 1头）进行猪肺炎支原体（Mhp）、副猪嗜血杆菌（Hps）和猪胸膜肺炎放线杆菌（App）PCR检测,进而进行细菌的分离鉴定和药敏试验。结果显示：4份病料中App和Mhp均为阴性,Hps在猪场A和C的病料中为阴性,猪场B病料中为阳性;在猪场A分离出奇异变形杆菌和猪链球菌各1株;猪场B分离出1株化脓隐秘杆菌、1株嗜麦芽窄食单胞菌和1株奇异变形杆菌;猪场C分离出1株化脓隐秘杆菌和1株奇异变形杆菌。药敏试验结果显示：化脓隐秘杆菌对诺氟沙星、洛美沙星敏感,嗜麦芽窄食单胞菌对恩诺沙星敏感,奇异变形杆菌对诺氟沙星、恩诺沙星敏感,猪链球菌对诺氟沙星、洛美沙星、环丙沙星、氧氟沙星敏感。小鼠致病性试验结果表明：变形奇异杆菌、嗜麦芽窄食单胞菌和化脓隐秘杆菌均能使小鼠出现一过性精神萎靡;猪链球菌引起试验小鼠全部死亡,具有较强致病力。Porcine respiratory disease complex （PRDC） is caused by a variety of pathogenic factors. Pathogens of the disease includes viruses, bacteria, parasites, etc. , of which bacteria are the role factors caused the PRDC. Many bacterial pathogens can cause the disease,different prevention and treatment measures for different bacteria should be taken. 4 lungs of PRDC pigs were obtained from 3 pigs farms （marked A,B and C farms,1 diseased pig from A,2 from B,1 from C）,Myco- plasma pneumoniae （Mhp）,Haemophlius parasuis （Hps）and Pneumocystis aeruginosa （App） were detected by PCR in lungs first, furthermore, the bacteria were isolated and identified, the drug sensitive test of the isolates also were carried out in this study. The results showed that App and Mph were undetectable in the 5 lungs, Hps was undetectable in the lungs from A and C pig farms, but Hps was detectable in 1 lungs of B farm;two kinds of pathogenic bacteria （Proteus mirabilis and Streptococcus suis） were obtained in A farm;three kinds of bacteria （Trueperella pyogenes, Stenotrophomonas maltophilia and Proteus mirabilis） obtained in B farm; two kinds of bacteria （Trueperella pyogenes and Proteus mirabilis） obtained in C farm. Drug sensitivity test results showed that the Trueperella pyogenes is sensitive to norfloxacin, Stenotrophomonas maltophilia is sensitive to enrofloxacin, Proteus mirabis is sensitive to norfloxacin and enrofloxacin, Strepto- coccus suis is sensitive to norfloxacin, lomefloxacin, ciprofloxacin and ofloxacin. The isolatesinfection test of mouse omonas maltophilia ca Streptococcus suis isola showed that the Trueperella pyogenes ,Proteus mirabilis and Stenotroph- used the clinical symptoms such as depressed, anorexia and trembling. The te is virulent to experimental mice.

关 键 词：猪 猪呼吸道疾病综合征 细菌 分离鉴定 药敏试验 

分 类 号：S852.61[农业科学—基础兽医学]