普通野生稻根特异启动子的克隆与鉴定  

Cloning and Identification of a Promoter with Root Tissue-specific Expression in Common Wild Rice

作　　者：黄珂 黄格格 薛满德 龙艳[2] 袁潜华 裴新梧[2] HUANG Ke;HUANG Ge-ge;XUE Man-de;LONG Yan;YUAN Qian-hua;PEI Xin-wu(College of Tropical Agriculture and Forestry,Hainan University,Haikou 570228;MOA Key Laboratory on Safety Assessment（Molecular）of Agri-GMO,Chinese Academy of Agricultural Sciences,Beifing 100081)

机构地区：[1]海南大学热带农林学院,海口570228 [2]农业部转基因安全评价重点实验室中国农业科学院生物技术研究所,北京100081

出　　处：《生物技术通报》2018年第8期87-92,共6页Biotechnology Bulletin

基　　金：转基因重大专项(2016ZX08011-001);国家自然科学基金项目(31260153)

摘　　要：通过普通野生稻转录组文库筛选到一个普通野生稻(Oryza rufipogon Griff.)根特异表达基因,并克隆启动子序列,命名为Or RSGp,长度为896 bp,含有CAAT-box、TATA-box等主要功能元件,还有参与茉莉酸甲酯反应的响应元件,以及抗旱、抗逆等响应元件。将其与gus报告基因融合转化拟南芥,GUS组织化学染色与定量分析结果表明,Or RSGp调控gus基因在根部特异表达。Based on previous transcriptome data, a root-specific expression gene of common wild rice （ Oryza rufipogon Griff. ） was screened, and the promoter sequence was cloned and designated as OrRSGp. It was 896 bp in length and contained main functional elements such as CAAT-box and TATA-box, MeJA-responsiveness and some elements involved in defense and stress responsiveness. It was fused with the β-glucuronidas （ gus ） reporter gene and transformed into Arabidopsis thaliana. GUS histochemical staining and quantitative analysis showed that OrRSGp regulated gene gus specific expression in roots.

关 键 词：普通野生稻 启动子 根特异表达 

分 类 号：S511.9[农业科学—作物学]