五指山小型猪-藏酋猴异种肝移植的术前检测  

Pre-operative detection of Wuzhishan miniature pig-Tibetan monkey xenotransplantation

作　　者：白鸽 李霄 张虹 黄敏 陶开山 窦科峰 Bai Ge;Li Xiao;Zhang Hong;Huang Min;Tao Kaishan;Dou Kefeng(Department of Hepatobiliary Surgery,Xijing Hospital,Air Force Military Medical University,Xi＇an 710032,Shaanxi,China)

机构地区：[1]空军军医大学西京医院肝胆外科全军器官移植研究所,陕西西安710032

出　　处：《实用器官移植电子杂志》2018年第5期355-358,共4页Practical Journal of Organ Transplantation(Electronic Version)

基　　金：国家"973"计划基金资助项目(2015CB554100);国家重点研发计划资助项目(2017YFC1103703);国家"863"计划基金资助项目(2012AA021005);国家自然科学基金资助项目(81300361;81270549;81670593;81470873;81671838);陕西省自然科学基础研究计划资助项目(2017JM8014);陕西省科技统筹创新工程计划资助项目(2012KTCL03-01;2011KTCL03-15);西京医院学科助推计划基金资助项目(XJZT12M09;XJZT13Z01;XJZT14Z04)

摘　　要：目的本研究为了减少五指山小型猪-藏酋猴异种肝移植手术的超急性排斥反应(hyperacute rejection,HAR)和急性排斥反应(acute rejection,AR)发生率,进行全面术前检测。方法分别利用PCR技术和植物凝集素细胞染色对供体GGTA1基因敲除类型进行鉴定;30只藏酋猴分别与五指山小型猪进行淋巴细胞毒实验;供受体ABO血型鉴定。结果供体为GGTA1基因敲除猪,GTKO猪外周血单个核细胞(peripheral blood mononuclear cell,PBMC)植物凝集素细胞染色未检测出荧光,表明GGTA1基因敲除成功,与基因型鉴定结果一致。通过淋巴细胞毒实验选定28#与供体PBMC毒性最小的藏酋猴做为备选受体。血型鉴定显示供受体血型一致。结论通过对GTKO五指山小型猪-藏酋猴异种肝移植的术前检测选定合适的受体,可有效减少异种移植HAR和AR的发生率。Objective To perform comprehensive preoperative testing in order to reduce the risk of hyperacute rejection （HAIR） and acute rejection （AIR） in the Wuzhishan miniature pig-Tibetan monkey xenotransplantation. To Minimize the influence of immunological factors on recipients survival in order to prolong the recipients survival. Methods The donor GGTA1 gene knockout type was identified by PCIR and lectin cell staining, respectively. A total number of 30 Tibetan monkey and Wuzhishan miniature pigs were tested for lymphocyte toxicity and ABO blood group identification. Results The donor was GGTA1 knockout pigs, and the fluorescence was not detected in GTKO pig peripheral blood mononuclear cell （PBMC） with lectin cell staining, it indicated that the GGTA1 gene was successfully knocked out, which was consistent with the genotype identification results. The Tibetan monarch monkey 28# with the least toxicity to the donor PBMC through lymphocyte toxicity test was selected as a candidate recipient. Blood type test showed identical blood type between donor and recipients. Conclusion The preoperative test of GTKO Wuzhishan miniature pigs-Tibetan monkey xenotransplantation has greatly reduced the risk of HAIR and AIR after xenotransplantation.

关 键 词：异种肝移植 α-1 3-半乳糖苷转移酶 五指山小型猪 藏酋猴 淋巴细胞毒实验 

分 类 号：R657.3[医药卫生—外科学]