线粒体保护剂SS-31对发育期大鼠异氟醚麻醉后海马线粒体形态和认知功能的影响  

Effects of mitochondrial antioxidant SS-31 on hippocampal mitochondrial morphology and cognition after isoflurane-anesthesia in developing rats

作　　者：付群[1] 石金云[1] 张慧[2] 邱丽丽[2] 吴晶 Fu Qun1, Shi Jinyun1, Zhang Hui2, Qiu Lili2, Wu Jing2(1Department of Anesthesiology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028; 2Department of Anethesiology, ZhongDa Hospital Affiliated to Southeast University, Nanjing 210009, China)

机构地区：[1]南京中医药大学附属中西医结合医院麻醉科,江苏南京210028 [2]东南大学附属中大医院麻醉科,江苏南京210009

出　　处：《南京医科大学学报（自然科学版）》2018年第9期1204-1207,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81600950)

摘　　要：目的:观察线粒体靶向抗氧化剂SS-31对异氟醚麻醉发育期大鼠海马线粒体形态和认知功能的影响。方法:新生鼠出生后(postnatal day,PND)第7天,随机均分为3组(n=24):氧气+生理盐水组(CN组)、异氟醚+生理盐水组(IN组)和异氟醚+SS-31组(IS组)。根据分组,于氧气或异氟醚吸入前30 min腹腔注射等容生理盐水或SS-31(5 mg/kg),吸入氧气或异氟醚6 h。分别于气体吸入6 h后即刻(PND7)检测海马组织活性氧自由基(reactive oxygen species,ROS)和丙二醛(malondialdehyde,MDA)水平及超氧化物歧化酶(superoxide dismutase,SOD)活性;PND21检测海马组织线粒体形态;PND40、PND60进行旷场实验和水迷宫实验检测大鼠行为学表现。结果:与IN组比较,IS组ROS和MDA水平降低,SOD活性升高;异常线粒体数量减少;PND60测试阶段穿越平台次数和目标象限时间明显增多。结论:线粒体靶向抗氧化剂SS-31可以抑制异氟醚麻醉所致发育期大鼠海马氧化应激反应的增强,具有改善线粒体形态和认知功能的作用。Objective：To observe the effects of mitochondrion-targeted antioxidant SS-31 on hippocampal mitochondrial morphology and cognition after isoflurane-anesthesia in developing rats. Methods：Seventy-two postnatal day （PND）7 rats were equally divided into 3 groups （n=24） ：oxygen ＋ normal saline （the CN group） , isoflurane ＋ normal saline （the IN group） , and isoflurane ＋ SS-31 （the IS group）. SS-31 （5 mg/kg）or normal saline was intraperitoneally administered with a volume of 0.4 mL/kg 30 min before gas inhalation. Malondialdehyde （MDA）content, reactive oxygen species （ROS）levels, and superoxide dismutase （SOD）activities were determined on PNDT. Mitoehondrial morphology was determined on PND21, and behavioral studies were performed on PND40 or PND60. Results： Compared with the IN group, the levels of ROS and MDA, and number of abnormal mitochondria were decreased, while the activity of SOD, times of crossingplatform and time spending in target quadrant were increased in the IS group. Conclusion： Mitochondrial antioxidant SS- 31 attenuates isofluraneinduced oxidative stress, and impairments of mitoehondrial morphology and cognition in developing rats.

关 键 词：线粒体 异氟醚 抗氧化剂 认知 发育期大鼠 

分 类 号：R614.2[医药卫生—麻醉学]