显微注射法利用胚胎干细胞制备嵌合鼠的研究  

The Study of Generation Mouse Chimeras from Embryonic Stem Cell by Microinjection Method

作　　者：马玉娥 MaYu-e(Cam-Su Genomic Resource Center Soochow University,JiangsuSuzhou 215123)

机构地区：[1]苏州大学剑桥-苏大基因资源中心,江苏苏州215123

出　　处：《生物化工》2018年第5期144-146,158,共4页Biological Chemical Engineering

摘　　要：嵌合鼠模型主要用于基因功能、胚胎发育、细胞分化、调控机制和致病机理等方面的研究。嵌合鼠的制备方法主要有显微注射法、聚合法和共培养法。本文主要对嵌合鼠制备的最常用方法—显微注射法及其嵌合体制备的影响因素作一综述,为研究者在高效获取嵌合体方面提供参考。Chimeric mice model is mainly applied to study gene function, embryo development, cell differentiation, development mechanisms and disease pathogenesis and so on. There are three methods for producing chimeras： mieroinjeetion, aggregation and coculturing. This review is mainly to introduce the most common method of generation chimeras-microinjection and influencing factors of generation chimeras. It aims to provide referencesfor researchers to produce chimerasefficiently.

关 键 词：显微注射 胚胎干细胞 嵌合鼠 

分 类 号：Q813.2[生物学—生物工程]