半边莲生物碱类物质鉴定及对Hela细胞抑制作用研究  

Research of alkaloids identification in Lobelia and Inhibition of Hela cells

作　　者：孙尧[1] 张皓[2] 孙佳明[3] 张辉[3] 高晓晨[3] SUN Yao;ZHANG Hao;SUN Jiaming;ZHANG Hui;GAO Xiaochen(Changchun University of Technology,Changchun 130012,China;Jilin Provincial College of Economic Management Cadres,Changchun 130012,China;Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春工业大学,长春130012 [2]吉林省经济管理干部学院,长春130012 [3]长春中医药大学,长春130117

出　　处：《吉林中医药》2018年第9期1078-1081,共4页Jilin Journal of Chinese Medicine

基　　金：吉林省科技厅重大科技项目(20150203005YY);吉林省教育厅科学技术研究(吉教科合字【2016】第29号)

摘　　要：目的本研究首次通过乙醇回流提取法及正丁醇萃法,对半边莲中的主要生物碱成分进行提取。方法应用超高效液相-串联质谱联用技术UPLC-MS技术及电喷雾串联质谱技术ESI-MS2的方法对半边莲中主要生物碱结构分析。结果通过高速逆流色谱HSCCC提取出新山梗菜碱B和新山梗菜碱A两种生物碱单体,MTT法检测山梗菜总提取物,及新山梗菜碱B和新山梗菜碱A两种生物碱分别对Hela细胞培养24,48,72 h的抑制作用。结论半边莲中的生物碱对Hela细胞癌症模型起抑制作用。Objective Ethanol reflux extraction and n-butanol extraction were used for extracting alkaloids from Lobelia in this study. Methods Ultra performance liquid chromatography tandem mass spectrometry UPLC-MS combined with electrospray tandem mass spectrometry ESI-MS2 methods was analysed for structure of the main alkaloids in Lobelia. Results New Lobeline A and New Lobeline B was refined and extracted used High speed counter current chromatography HSCCC. The detection of total extract of Lobelia MTT, and Johor stem vegetable base B and a base A of two alkaloids in Pakchoi were 24, 48, 72 h of Hela cells. Conclusion The alkaloids of Scutellaria hematidis can inhibit the cancer model of Hela cells.

关 键 词：半边莲 UPLC-MS HELA细胞 

分 类 号：R284[医药卫生—中药学]