大黄对氧应激诱导大鼠新型支气管肺发育不良中热休克蛋白70表达的调控及肺保护作用  被引量:1

Regulation of rhubarb on heat shock protein 70 expression and lung protection in rats with hyperoxiainduced new bronchopulmonary dysplasia

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作  者:郭亮 叶贞志 陈晓利 唐丽君[2] 黄为民[2] 宋小燕[2] Guo Liang;Ye Zhenzhi;Chen Xiaoli;Tang Lijun;Huang Weimin;Song Xiaoyan(Neonatology Department,Guangming New District People's Hospital,Shenzhen 518106,China)

机构地区:[1]深圳市光明新区人民医院新生儿科,518106 [2]南方医科大学南方医院新生儿科,广州510515

出  处:《中国小儿急救医学》2018年第9期683-686,共4页Chinese Pediatric Emergency Medicine

基  金:广东省省级科技计划项目(2014A020212526)

摘  要:目的探讨大黄对氧应激导致的大鼠新型支气管肺发育不良(BPD)的影响。方法足月顺产新生SD大鼠96只按随机数字表法分入对照组(空气组)、BPD组(新型BPD动物模型组)、大黄组(新型BPD动物模型+大黄干预组)及热休克蛋白70(HSP70)表达拮抗组(新型BPD动物模型+大黄干预+HSP70表达拮抗组)。实验组大鼠从第4天起暴露在600 ml/L的氧环境中(对照组置于空气环境中),于实验第5天始进行大黄或者大黄和HSP70表达拮抗剂槲皮素处理,分别对给药后7 d、14 d及21 d大鼠的肺组织进行免疫染色分析,并用免疫印记法对其HSP70进行定量分析。结果(1)与对照组相比,BPD组大鼠肺组织发生类似新型BPD的病理改变,大黄的存在(大黄组)会抑制肺泡的病变。槲皮素能够有效抑制HSP70的表达,在大黄和槲皮素同时存在的情况下(HSP70表达拮抗组)肺组织仍然出现类似新型BPD病理改变。(2)BPD组及HSP70表达拮抗组大鼠实验后14 d、21 d放射状肺泡计数均明显低于对照组及大黄组大鼠,差异均有统计学意义(F=27.96,P〈0.01;F=70.87,P〈0.01)。(3)大黄组大鼠给药后14 d及21 d肺组织HSP70表达高于其他各组大鼠,差异均有统计学意义(F=62.46,P〈0.01;F=95.90,P〈0.01)。结论大黄可能通过促进HSP70的表达而对氧应激诱导的大鼠新型BPD起相应肺保护作用。ObjectiveTo investigate the effect of rhubarb on hyperoxia-induced new bronchopulmonary dysplasia (BPD) in rats.MethodsFull-term Sprague-Dawley rats were exposed or not (control group) in 600 ml/L oxygen 4 days after birth and injected with normal saline (BPD group), rhubarb (BPD+ rhubarb group) or a combination of rhubarb with quercetin (BPD+ rhubarb+ quercetin group). Immunohistochemical staining was used to detect the pathological changes of the rat lungs.HSP70 expression level was quantified by western blot.ResultsCompared with control group, BPD group showed decreased radial alveolar 14 and 21 days after the drug treatment, which was rescued by the coexistence of rhubarb.Quercetin, as an inhibitor of HSP70, counteracted the effect of rhubarb.The lung of the BPD+ rhubarb+ quercetin group showed a similar phenotypic change with that of the BPD group.In addition, the expressions of HSP70 in lung tissues of rhubarb group at 14 days and 21 days after the treatment were higher than those of other groups, there were significant differences between rhubarb group and other groups(F=62.46, P〈0.01; F=95.90, P〈0.01).ConclusionRhubarb may attenuate the hyperoxia-induced new bronchopulmonary dysplasia in rats by activating HSP70 expression.

关 键 词:热休克蛋白70 支气管肺发育不良 大黄 氧应激 

分 类 号:R722.1[医药卫生—儿科] R-332[医药卫生—临床医学]

 

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