通过酒精饲养小鼠建立小鼠酒精模型  

Mice Alcohol Model Establishment by Feeding Mice with Alcohol

作　　者：汪冠豪 郭亮[1] 郑璐璐 许东坡 刘箐[1] WANG Guan-hao;GUO Liang;ZHENG Lu-lu;XU Dong-po;LIU Qing(Schl.of Med.Inst.＆ Food Engin.,Uni.of Shanghai for Sci.＆ Technol.,Shanghai 20009)

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093

出　　处：《微生物学杂志》2018年第5期90-95,共6页Journal of Microbiology

基　　金：国家自然科学基金项目(31371776)

摘　　要：研究通过酒精饲养小鼠建立小鼠酒精成瘾模型的方法。使用6周龄SPF级Balb/c雄性小鼠15只,随机分为3组(n=5),即灌胃和酒水喂养组(A)、灌胃组和水喂养组(B)、对照组(C)。3组固体饲料正常给予,A、B两组每日灌胃11%酒水200μL,A组酒水溶液自由摄取并作为唯一饮水来源,B组水自由摄取,C组不做处理。饲养8周后进行24 h酒精戒断,并进行矿场实验,强迫游泳实验。处死小鼠取肝脏、肾脏和结肠制作石蜡切片并观察。造模过程中3组小鼠进食、饮水、体重、内脏重量出现了不同程度的变化。行为学结果显示,矿场实验中A、B两组小鼠戒断酒精后相对对照组小鼠焦虑水平明显升高;强迫游泳实验中,A、B两组相对C组绝对不动时间显著加长,表现出精神抑郁。显微镜观察小鼠内脏石蜡切片,A组小鼠肝脏切片明显出现肝细胞排列紊乱,肾脏中肾小球上皮细胞轻度萎缩,空泡变性,肾小管无明显病变,结肠石蜡切片肠绒毛出现破损,肠绒毛顶端完整性降低。B组肝、肾、结肠石蜡切片与对照组相比无明显变化。The establishment of a mice model be addicted to alcohol by feeding mice with alcohol was studied. Fifteen male Balb/c mice of 6 weeks old at SPF grade were adopted, and randomly divided into 3 groups （n=5）, i.e. intragastric and alcoholic beverage feeding group （A）, intragastric group and water feeding group （B）, and control group （C）. The three groups were fed normally with solid forage. Both group A and B were fed intragastrically with 200 μL of 11% alcoholic water daily. Group A was free to take alcohol solution as the only source of drinking water. Group B was free to take water, and group C was not treated. Eight weeks after the feeding, then carried out alcoholic withdrawal for 24 h, and field experiments, forced swimming experiment. The mice were sacrificed to take their liver, kidney and colon and prepared paraffin sections for observation. During the model-making process of the three groups of mice, their food taking, drinking, weight, and visceral weight appeared changes in varying degree. The results of behavioral study showed that anxiety levels of mice in group A and group B in field test were significantly higher than those in control group after abstinence from alcohol mines. In forced swimming test, absolute immobility time of groups A and B relative to group C was significantly longer, and assumed mental depression. The visceral paraffin sections of mice were observed under microscope. The liver sections of mice in Group A appeared liver cells disorder, the glomerular epithelial cells in kidney were mildly atrophy, and vacuolar degeneration, the kidney tubules showed no obvious pathology, the intestinal villus in the paraffin sections of colon appeared damage, and the integrity of intestinal villous apex decreased. Paraffin sections of liver, kidney, and colon in Group B had no significant change as compared with control group.

关 键 词：酒精成瘾 小鼠模型 动物行为学 石蜡切片 

分 类 号：Q93-33[生物学—微生物学]