HPLC双波长法同时测定炎热清片中6种有效成分的含量  

Simultaneous Determination of Six Components in Yanreqing Tablets by Double-wavelength HPLC

作　　者：邓俊杰 DENG Junjie(Shaoxing Institute for Food and Drug Control,Shaoxing 312088,China)

机构地区：[1]绍兴市食品药品检验研究院,浙江绍兴312088

出　　处：《中国现代应用药学》2018年第9期1329-1332,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立HPLC双波长法同时测定炎热清片中龙胆苦苷、栀子苷、芒果苷、黄芩苷、黄芩素和汉黄芩素6种成分的含量。方法采用Agilent TC-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇∶0.2%磷酸水溶液(梯度洗脱),柱温为35℃,流速为1.0 mL·min^(-1),检测波长0~20 min为254 nm,同时检测龙胆苦苷、栀子苷、芒果苷,20~50 min为280 nm,同时检测黄芩苷、黄芩素、汉黄芩素。结果龙胆苦苷、栀子苷、芒果苷、黄芩苷、黄芩素、汉黄芩素分别在18.92~189.2 ng(r=0.999 9),107.44~1 074.4 ng(r=0.999 9),9.82~98.2 ng(r=0.999 9),767.68~7 676.8 ng(r=1.000 0),100.94~1 009.4 ng(r=0.999 9),49.84~498.4 ng(r=0.999 9)线性良好,平均回收率(n=9)分别为98.31%,98.35%,98.40%,98.03%,98.46%,98.24%,RSD分别为0.4%,0.3%,0.3%,0.4%,0.4%,0.3%。结论该方法灵敏、简便、准确,可用于炎热清片的质量控制。OBJECTIVE To establish a new double-wavelength HPLC method for simultaneous determination of 6 main components in Yanreqing tablets. METHODS The method was performed on an Agilent TC-C_（18） column（4.6 mm×250 mm, 5 μm）. The mobile phase was consisited of methanol-0.2% phosphoric acid by gradient elution at 35 column temperature.℃ The flow rate was 1.0 mL·min（-1）. The detection wavelength was 254 nm for gentiopicroside, geniposide, mangiferin at 0-20 min, and 280 nm for baicalin, baicalein, wogonin at 20-50 min. RESULTS Gentiopicroside, geniposide, mangiferin, baicalin, baicalein, wogonin showed a good linear relationship in the range of 18.92-189.2 ng（r=0.999 9）, 107.44-1 074.4 ng（r=0.999 9）, 9.82-98.2 ng（r=0.999 9）, 767.68-7 676.8 ng（r=1.000 0）, 100.94-1 009.4 ng（r=0.999 9）, 49.84-498.4 ng（r=0.999 9）, respectively. The average recoveries were 98.31%, 98.35%, 98.40%, 98.03%, 98.46%, 98.24%, the RSD were 0.4%, 0.3%, 0.3%, 0.4%, 0.4%, 0.3%. CONCLUSION The method is sensitive, simple, accurate, and suitable for the quality control of Yanreqing tablets.

关 键 词：炎热清片 龙胆苦苷 栀子苷 芒果苷 黄芩苷 黄芩素 汉黄芩素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]