淫羊藿总黄酮胶囊中黄酮类成分含量测定及抗骨质疏松活性研究  

Determination of flavonoids constituents in epimedium total flavone capsule and research on its anti-osteoporosis activity

作　　者：徐忠坤[1,2,3] 殷洪梅 李芳[1,2,3] 秦建平 顾少莉[1,2,3] 吴云 王振中[1,2,3] 萧伟 XU Zhong-kun;YIN Hong-mei;LI Fang;QIN Jian-ping;GU Shao-li;WU Yun;WANG Zhen-zhong;XIAO Wei(Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China;State Key Laboratory of Pharmaceutical Process New-tech for Chinese Medicine,Lianyungang 222001,China;Key Laboratory for the New Technique Research of Traditional Chinese Medicine Extraction and Purification,Lianyungang 222001,China)

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001 [3]中药提取精制新技术重点研究室,江苏连云港222001

出　　处：《中国中药杂志》2018年第15期3140-3144,共5页China Journal of Chinese Materia Medica

基　　金：国家“重大新药创制”科技重大专项(2013ZX09402203)

摘　　要：采用快速液相色谱法（RRLC）对淫羊藿总黄酮胶囊进行含量测定,建立了淫羊藿新苷A、朝霍定A1、朝霍定A、朝霍定B、朝霍定C、淫羊藿苷、宝藿苷Ⅱ、淫羊藿次苷Ⅰ、箭藿苷B、鼠李糖基淫羊藿次苷Ⅱ、宝藿苷Ⅰ等11个黄酮类成分的含量测定方法。同时,通过检测碱性磷酸酶（AKP）含量,考察了11个化合物诱导MC3T3-E1细胞增殖分化的效果,结果显示宝藿苷Ⅱ活性最好,宝藿苷Ⅱ和淫羊藿次苷Ⅰ活性都大于淫羊藿苷。该研究建立了黄酮苷类成分的含量测定方法,并比较了每个单体抗骨质疏松的效果,为淫羊藿总黄酮胶囊药效物质基础和作用机制研究提供了技术支持。To develop a rapid resolution liquid chromatography（RRLC） method for the simultaneous determination of epimedoside A,epimedin A1,epimedin A,epimedin B,epimedin C,icariin,baohuosideⅡ,icarisideⅠ,sagittatoside B,2″-O-rhamnosyl icarisideⅡ,and baohuosideⅠin epimedium total flavone capsule. At the same time,the effects of the above 11 compounds on osteogenic differentiation of MC3 T3-E1 cells were investigated by detecting the content of alkaline phosphatase（AKP）. The results showed that baohuoside Ⅱ had the highest activities,and both the activities of baohuoside Ⅱ and icariside Ⅰ were stronger than those of icariin.In this study,the content determination method of flavonoid glycosides was established,and the anti-osteoporosis effect of monomers was compared,providing technical support for the study of the pharmacodynamic and mechanism of Epimedium total flavone capsule.

关 键 词：淫羊藿总黄酮胶囊 黄酮苷 RRLC MC3T3-E1 AKP 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]