宁肠汤对肠易激综合征大鼠结肠黏膜IL-1β表达影响的实验研究  

Experimantal Study on Effect of Ningchang Tang(宁肠汤) on IL-1β Expression in Colic Mucosa of Irritable Bowel Syndrome Rats

作　　者：杨江升 章宏[2] 黄志群 汪霖 熊礼凤 李平儿 YANG Jiangsheng;ZHANG Hong;HUANG Zhiqun;WANG Lin;XIONG Lifeng;LI Ping＇er(First Hospital of Fuyang District,Hangzhou,Zhejiang 311400;First Affiliated Hospital of Zhejiang Chinese Medicine University,Hangzhou,Zhejiang 310006)

机构地区：[1]浙江省杭州市富阳区第一医院,浙江杭州311400 [2]浙江大学医学院附属第一医院,浙江杭州310000

出　　处：《中国中医药科技》2018年第5期641-643,共3页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省杭州市富阳区科技局项目(2014SK014)

摘　　要：目的:观察宁肠汤对肠易激综合征模型大鼠结肠黏膜炎性细胞因子IL-1β表达的影响,探讨宁肠汤的作用机制。方法:将60只雄性SD大鼠随机分为正常对照,模型对照,得舒特和宁肠汤低、中、高浓度组,建立束缚应激致肠易激综合征模型,分组给药14 d,并免疫组化法检测大鼠结肠黏膜IL-1β表达。结果:与正常组比较,模型组大鼠结肠黏膜IL-1β表达明显升高(P<0.01);各给药组大鼠结肠黏膜IL-1β表达明显降低(P<0.01);高浓度宁肠汤组相对低浓度宁肠汤组IL-1β表达量降低显著(P<0.05)。结论:宁肠汤可能通过降低IBS大鼠结肠黏膜炎性因子IL-1β表达而调节胃肠功能,治疗肠易激综合征。Objective： To explore the mechanism of Changnbing Tang on irritable bowel syndrome（ IBS） by observing its effect on IL-1βexpression in colic mucosa of IBS rats. Methods： 60 male SD rats were divided into normal,model,Pinaverium Bromide and Ningchang Tang low,midddle,high dose groups,IBS models were induced by tail-clamping on the basis of modified Williams method,each group was given the corresponding medicine or saline for 14 days. IL-1 βexpression in colic mucosa was determined by immunohistochemistry method. Results： Compare with normal group,the IL-1βexpression in colic mucosa of model group was raised significantly（ P〈0. 01）; the IL-1βexpression in colic mucosa was lowered in each medicine group（ P〈0. 01）,the effect of high dose group was more significant than that of low dose group（ P〈0. 05）. Conclusion：Ningchang Tang can regulate intestinal function of IBS rats by decreasing IL-1βexpression in colic mucosa.

关 键 词：宁肠汤 肠易激综合征 结肠黏膜 白细胞介素-1Β 大鼠 

分 类 号：R285.5[医药卫生—中药学]