基于死亡受体途径探讨脾肾双补方对离体人早孕绒毛滋养细胞HTR-8凋亡蛋白TNF-α、Caspase-3的影响  

Investigation on Influence of Pishen Shuangbu Formula on TNF-α and Caspase-3 of Human Trophoblast Cells HTR-8 in Vitro Culture During Early Pregnancy Through Based on Apoptosis Receptor Pathway

作　　者：曾倩[1] 薛华容 邓礼林 夏宛廷 周航[2] 李宛静 彭尧[2] 谢佳 王毅[1] ZENG Qian;XUE Huarong;DENG Lilin;XIA Wanting;ZHOU Hang;LI Wanjing;PENG Yao;XIE Jia;WANG Yil(Chengdu University of TCM Affiliated Hospital,Chengdu 610075,Sichuan,China;Clinical Medicine School of Chengdu University of TCM,Chengdu 610072,Sichuan,China)

机构地区：[1]成都中医药大学附属医院,四川成都610075 [2]成都中医药大学临床医学院,四川成都610072

出　　处：《中华中医药学刊》2018年第10期2311-2314,I0001-I0003,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：成都市科技局项目(2014-HM01-00318-SF)

摘　　要：目的：探索脾肾双补方对人早孕绒毛滋养细胞中凋亡相关蛋白TNF-α、Caspase-3表达的影响。方法：采用动物含药血清,体外分组培养HTR-8细胞48 h,采用免疫组织化学法检测各组细胞凋亡蛋白TNF-α、Caspase-3表达的情况。结果：与空白对照组比,脾肾双补方高、中剂量组HTR-8细胞凋亡蛋白TNF-α、Caspase-3的表达均降低（P 〈0. 05）,地屈孕酮组HTR-8细胞TNF-α、Caspase-3的表达显著低于空白对照组（P 〈0. 01）;与地屈孕酮组比,脾肾双补方高、中剂量组TNF-α、Caspase-3的表达差异无统计学意义（P〉0. 05）;脾肾双补方高、中剂量组间TNF-α的表达差异无统计学意义（P〉 0. 05）。结论：脾肾双补方含药血清可以促进人早孕绒毛滋养细胞数目增加,其机制可能是：减少人早孕绒毛滋养细胞的凋亡,保护细胞存活,继而有利于早期妊娠的维持,其作用途径可能是：脾肾双补方含药血清通过降低细胞凋亡诱导蛋白TNF-α的表达,抑制死亡受体途径,减少凋亡执行蛋白Caspase-3的表达,从而抑制人早孕绒毛滋养细胞的凋亡,保护细胞存活,维持妊娠。Objective： To investigate the effect of Pishen Shuangbu Formula（ PSSB） on the proteins expression of TNF-α and Caspase-3 in human chorionic trophoblast cells. Method： The HTR-8 cells were cultured in vitro for 48 h using animal-containing serum. And the expressions of TNF-α and Caspase-3 in HTR-8 cells were detected by immunohistochemistry. Results： Compared with the blank control group,the expressions of TNF-α and Caspase-3 in HTR-8 cells in PSSB high and medium dose groups were decreased（ P〈0. 05）,and the dydrogesterone group＇s was significantly lower than that of the blank control group（ P〈0. 01）. Compared with the dydrogesterone group,the expressions of TNF-α and Caspase-3 in HTR-8 cells in PSSB high and medium dose groups had no significant difference（ P〉0. 05） and there was no significant difference in the expression of TNF-α between the middle and high dose groups（ P〉0. 05）. Conclusion：PSSB medicated serum can promote human pregnancy villus sertoli cells numbers. Its mechanism might be reducing pregnancy villi of sertoli cell apoptosis,protecting cells survival and conducive to the maintenance of early pregnancy. The role of approaches may be the PSSB medicated serum induced by reducing cell apoptosis protein expression of TNF-α,inhibition of death receptor pathway,reducing apoptosis protein expression of Caspase-3,and thus inhibiting human pregnancy villus sertoli cell apoptosis,protecting cells survive and maintain pregnancy.

关 键 词：脾肾双补方 HTR-8细胞 TNF-Α CASPASE-3 先兆流产 

分 类 号：R285.5[医药卫生—中药学]